Identification of novel microRNA-like molecules generated from herpesvirus and host tRNA transcripts

Tiffany A. Reese, Jing Xia, L. Steven Johnson, Xiang Zhou, Weixiong Zhang, Herbert W. Virgin

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We applied deep sequencing technology to small RNA fractions from cells lytically infected with murine gammaherpesvirus 68 (γHV68) in order to define in detail small RNAs generated from a cluster of tRNA-related polycistronic structures located at the left end of the viral genome. We detected 10 new candidate microRNAs (miRNAs), six of which were confirmed by Northern blot analysis, leaving four as provisional. In addition, we determined that previously identified and annotated viral miRNA molecules were not necessarily represented as the most abundant sequence originating from a transcript. Based on these new small RNAs and previously reported γHV68 miRNAs, we were able to further describe and annotate the distinctive γHV68 tRNA-miRNA structures. We used this deep sequencing data and computational analysis to identify similar structures in the mouse genome and validated that these host structures also give rise to small RNAs. This reveals a possible convergent usage of tRNA/polymerase III (pol III) transcripts to generate small RNAs from both mammalian and viral genomes.

Original languageEnglish (US)
Pages (from-to)10344-10353
Number of pages10
JournalJournal of Virology
Volume84
Issue number19
DOIs
StatePublished - Jan 1 2010

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Herpesviridae
Transfer RNA
MicroRNAs
microRNA
RNA
High-Throughput Nucleotide Sequencing
Viral Genome
genome
Murid herpesvirus 4
Northern blotting
Northern Blotting
Genome
Technology
mice
cells
high-throughput nucleotide sequencing

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Identification of novel microRNA-like molecules generated from herpesvirus and host tRNA transcripts. / Reese, Tiffany A.; Xia, Jing; Johnson, L. Steven; Zhou, Xiang; Zhang, Weixiong; Virgin, Herbert W.

In: Journal of Virology, Vol. 84, No. 19, 01.01.2010, p. 10344-10353.

Research output: Contribution to journalArticle

Reese, Tiffany A. ; Xia, Jing ; Johnson, L. Steven ; Zhou, Xiang ; Zhang, Weixiong ; Virgin, Herbert W. / Identification of novel microRNA-like molecules generated from herpesvirus and host tRNA transcripts. In: Journal of Virology. 2010 ; Vol. 84, No. 19. pp. 10344-10353.
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