Identification of second and third calcitonin receptor-stimulating peptides in porcine brain

Takeshi Katafuchi, Kazumasa Hamano, Katsuro Kikumoto, Naoto Minamino

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We identified two cDNAs encoding new calcitonin receptor-stimulating peptides (CRSPs) in porcine hypothalamus cDNA library by cross-hybridization with the CRSP cDNA, and designated the second and third peptides as CRSP-2 and CRSP-3. The putative amino acid sequences of prepro-CRSP-2 and prepro-CRSP-3 showed higher identity with that of prepro-CRSP-1 than that of prepro-calcitonin gene-related peptide (CGRP), respectively, and these three CRSPs are considered to form a new family in the CGRP superfamily. RT-PCR analysis demonstrated that both CRSP-2 and CRSP-3 gene transcripts were expressed mainly in the central nervous system and thyroid gland. Synthetic CRSP-2 and CRSP-3 stimulated cAMP production very weakly in LLC-PK1 cells compared with CRSP and calcitonin (CT). Furthermore, CRSP-2 and CRSP-3 did not elicit a cAMP elevation at all in the COS-7 cells expressing CT receptor or CT-like receptor with or without one of receptor activity-modifying proteins. These results suggest the presence of still unidentified action mechanisms and functions of the peptides in the CGRP superfamily.

Original languageEnglish (US)
Pages (from-to)445-451
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume308
Issue number3
DOIs
StatePublished - Aug 29 2003

Keywords

  • Calcitonin
  • Calcitonin gene-related peptide
  • Calcitonin receptor
  • Calcitonin receptor-stimulating peptide
  • Calcitonin-like receptor
  • Receptor activity-modifying protein
  • cAMP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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