Identification of surface-exposed B-cell epitopes recognized by Haemophilus influenzae type b P1-specific monoclonal antibodies

H. Panezutti, O. James, E. J. Hansen, Y. Choi, R. E. Harkness, M. H. Klein, P. Chong

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A panel of P1 synthetic peptides was synthesized to map the surface- exposed epitopes of Haemophilus influenzae type b outer membrane protein P1 recognized by three murine monoclonal antibodies (MAbs 7C8, 3E12, and 6B1). By using peptide-specific enzyme-linked immunosorbent assays, MAbs 6B1, 7C8, and 3E12 were shown to recognize distinct epitopes localized within residues 60 to 88, 165 to 193, and 400 to 437 of mature P1, respectively. Since MAb 7C8 was shown previously to be protective against certain H. influenzae type b subtypes in the infant rat model of bacteremia, its cognate epitope was further characterized by using truncated peptide analogs. Fine mapping of the 7C8 epitope by competitive inhibition studies revealed that it was localized within residues 184 and 193.

Original languageEnglish (US)
Pages (from-to)1867-1872
Number of pages6
JournalInfection and immunity
Volume61
Issue number5
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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    Panezutti, H., James, O., Hansen, E. J., Choi, Y., Harkness, R. E., Klein, M. H., & Chong, P. (1993). Identification of surface-exposed B-cell epitopes recognized by Haemophilus influenzae type b P1-specific monoclonal antibodies. Infection and immunity, 61(5), 1867-1872.