Identification of T- and B-cell epitopes of the E7 protein of human papillomavirus type 16

Sarah A Comerford, Dennis J. McCance, Gordon Dougan, John P. Tite

Research output: Contribution to journalArticle

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Abstract

There is strong evidence implicating human papillomavirus type 16 (HPV16) in the genesis of human genital cancer. Viral DNA has been identified in invasive carcinoma of the uterine cervix and in cell lines derived from cervical carcinomas. These sequences are actively transcribed, and translation products corresponding to the early (E)-region genes have been identified. The most abundant viral protein is the E7 protein, which has been shown to possess transforming activity for both established and primary cells. In addition, it has been shown to bind to a cellular tumor suppressor, the retinoblastoma gene product (pRb-105). In view of these properties, we have undertaken the immunological analysis of this protein and have identified four T-cell epitopes and three B-cell epitopes by using a series of overlapping peptides spanning the entire HPV16 E7 sequence. Two of the B-cell epitopes were recognized by antisera from mice with three different murine (H-2) haplotypes (k, d, and s) immunized with two different E7 fusion proteins and from Fischer rats seeded with baby rat kidney cells transformed by HPV16 E7 and ras. A third B-cell epitope was recognized by antisera from CBA mice seeded with HPV16 E7-expressing L cells. Two regions of the protein contain common B- and T-cell epitopes, one of which appears to be particularly immunodominant.

Original languageEnglish (US)
Pages (from-to)4681-4690
Number of pages10
JournalJournal of Virology
Volume65
Issue number9
StatePublished - Sep 1991

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B-Lymphocyte Epitopes
T-Lymphocyte Epitopes
Human papillomavirus 16
Papillomaviridae
epitopes
B-lymphocytes
T-lymphocytes
Immune Sera
Proteins
proteins
antiserum
Retinoblastoma Genes
Carcinoma
mice
Inbred CBA Mouse
Inbred F344 Rats
Viral DNA
Viral Proteins
Tumor Suppressor Genes
Cervix Uteri

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of T- and B-cell epitopes of the E7 protein of human papillomavirus type 16. / Comerford, Sarah A; McCance, Dennis J.; Dougan, Gordon; Tite, John P.

In: Journal of Virology, Vol. 65, No. 9, 09.1991, p. 4681-4690.

Research output: Contribution to journalArticle

Comerford, Sarah A ; McCance, Dennis J. ; Dougan, Gordon ; Tite, John P. / Identification of T- and B-cell epitopes of the E7 protein of human papillomavirus type 16. In: Journal of Virology. 1991 ; Vol. 65, No. 9. pp. 4681-4690.
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