Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus

Tong Jin Zhao, Yang Liu, Yong Bin Yan, Feng Feng, Wei Qun Liu, Hai Meng Zhou

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We have previously identified two homologous groups of BnDREBs in Brassica napus, the trans-active BnDREBI and the trans-inactive BnDREBII, which provided an ideal system to study the trans-activation of DREB1/CBF. Deletion analysis indicated that the two additional regions in BnDREBI contributed little to the transcriptional activity. Domain swapping analysis indicated that all the domains contributed to the activity of BnDREBI, including the ERF/AP2 DNA binding domain. Through site-directed mutagenesis, we identified nine residues that were involved in the activity of BnDREBI, among which six residues are specific to BnDREBI, and three are common to DREB1A.

Original languageEnglish (US)
Pages (from-to)3044-3050
Number of pages7
JournalFEBS Letters
Volume581
Issue number16
DOIs
StatePublished - Jun 26 2007

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Keywords

  • Brassica napus
  • DRE
  • DREB1/CBF
  • Trans-activation
  • Transcriptional factors

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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