Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system

Carr D. Vincent, Jonathan R. Friedman, Kwang Cheol Jeong, Emily C. Buford, Jennifer L. Miller, Joseph P. Vogel

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Type IV secretion systems (T4SS) are utilized by a wide range of Gram negative bacteria to deliver protein and DNA substrates to recipient cells. The best characterized T4SS are the type IVA systems, which exhibit extensive similarity to the Agrobacterium VirB T4SS. In contrast, type IVB secretion systems share almost no sequence homology to the type IVA systems, are composed of approximately twice as many proteins, and remain largely uncharacterized. Type IVB systems include the Dot/Icm systems found in the pathogens Legionella and Coxiella and the conjugative apparatus of IncI plasmids. Here we report the first extensive characterization of a type IVB system, the Legionella Dot/Icm secretion apparatus. Based on biochemical and genetic analysis, we discerned the existence of a critical five-protein subassembly that spans both bacterial membranes and comprises the core of the secretion complex. This transmembrane connection is mediated by protein dimer pairs consisting of two inner membrane proteins, DotF and DotG, which are able to independently associate with DotH/DotC/DotD in the outer membrane. The Legionella core subcomplex appears to be functionally analogous to the Agrobacterium VirB7-10 subcomplex, suggesting a remarkable conservation of the core subassembly in these evolutionarily distant type IV secretion machines.

Original languageEnglish (US)
Pages (from-to)1278-1291
Number of pages14
JournalMolecular Microbiology
Volume62
Issue number5
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

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Legionella
Agrobacterium
Proteins
Coxiella
Membranes
Sequence Homology
Gram-Negative Bacteria
Molecular Biology
Membrane Proteins
Plasmids
Type IV Secretion Systems
DNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. / Vincent, Carr D.; Friedman, Jonathan R.; Jeong, Kwang Cheol; Buford, Emily C.; Miller, Jennifer L.; Vogel, Joseph P.

In: Molecular Microbiology, Vol. 62, No. 5, 01.12.2006, p. 1278-1291.

Research output: Contribution to journalArticle

Vincent, Carr D. ; Friedman, Jonathan R. ; Jeong, Kwang Cheol ; Buford, Emily C. ; Miller, Jennifer L. ; Vogel, Joseph P. / Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. In: Molecular Microbiology. 2006 ; Vol. 62, No. 5. pp. 1278-1291.
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