Identification of the predominant substrate for ADP-ribosylation by islet activating protein.

G. M. Bokoch, T. Katada, J. K. Northup, E. L. Hewlett, A. G. Gilman

Research output: Contribution to journalArticle

342 Citations (Scopus)

Abstract

Islet activating protein (IAP), a toxin isolated from Bordetella pertussis, blocks the ability of inhibitory hormones to attenuate adenylate cyclase activity and enhances the ability of stimulatory hormones to activate the enzyme. The toxin appears to act by catalyzing the transfer of ADP ribose from NAD to a 41,000-dalton protein in target cell membranes. A protein purified from rabbit liver membranes, apparently composed of 41,000- and 35,000-dalton subunits, is shown to be a specific substrate for IAP. Cholera toxin does not ADP-ribosylate this protein. In contrast, the purified guanine nucleotide-binding regulatory component of adenylate cyclase (G/F), which is ADP-ribosylated by cholera toxin, is not covalently modified by IAP. Equilibrium binding studies and photoaffinity labeling experiments demonstrate that the 41,000-dalton subunit of the IAP substrate has a specific binding site for guanine nucleotides.

Original languageEnglish (US)
Pages (from-to)2072-2075
Number of pages4
JournalJournal of Biological Chemistry
Volume258
Issue number4
StatePublished - Feb 25 1983

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Pertussis Toxin
Adenosine Diphosphate
Guanine Nucleotides
Cholera Toxin
Substrates
Adenylyl Cyclases
Hormones
Adenosine Diphosphate Ribose
Bordetella pertussis
Proteins
Cell membranes
Liver
NAD
Labeling
Binding Sites
Cell Membrane
Rabbits
Membranes
Enzymes
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bokoch, G. M., Katada, T., Northup, J. K., Hewlett, E. L., & Gilman, A. G. (1983). Identification of the predominant substrate for ADP-ribosylation by islet activating protein. Journal of Biological Chemistry, 258(4), 2072-2075.

Identification of the predominant substrate for ADP-ribosylation by islet activating protein. / Bokoch, G. M.; Katada, T.; Northup, J. K.; Hewlett, E. L.; Gilman, A. G.

In: Journal of Biological Chemistry, Vol. 258, No. 4, 25.02.1983, p. 2072-2075.

Research output: Contribution to journalArticle

Bokoch, GM, Katada, T, Northup, JK, Hewlett, EL & Gilman, AG 1983, 'Identification of the predominant substrate for ADP-ribosylation by islet activating protein.', Journal of Biological Chemistry, vol. 258, no. 4, pp. 2072-2075.
Bokoch GM, Katada T, Northup JK, Hewlett EL, Gilman AG. Identification of the predominant substrate for ADP-ribosylation by islet activating protein. Journal of Biological Chemistry. 1983 Feb 25;258(4):2072-2075.
Bokoch, G. M. ; Katada, T. ; Northup, J. K. ; Hewlett, E. L. ; Gilman, A. G. / Identification of the predominant substrate for ADP-ribosylation by islet activating protein. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 4. pp. 2072-2075.
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