Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi

David A. Lewis, Julia Klesney-Tait, Sheryl R. Lumbley, Christine K. Ward, Jo L. Latimer, Catherine A. Ison, Eric J. Hansen

Research output: Contribution to journalArticle

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Abstract

The znuA gene of Haemophilus ducreyi encodes a 32-kDa (mature) protein that has homology to both the ZnuA protein of Escherichia coli and the Pzp1 protein of H. infiuenzae; both of these latter proteins are members of a growing family of prokaryotic zinc transporters. Inactivation of the H. ducreyi 35000 znuA gene by insertional mutagenesis resulted in a mutant that grew more slowly than the wild-type parent strain in vitro unless ZnCl2 was provided at a final concentration of 100 μM. Other cations tested did not restore growth of this H. ducreyi mutant to wild-type levels. The H. ducreyi ZnuA protein was localized to the periplasm, where it is believed to function as the binding component of a zinc transport system. Complementation of the znuA mutation with the wild-type H. ducreyi znuA gene provided in trans restored the ability of this H. ducreyi mutant to grow normally in the absence of exogenously added ZnCl2. The wild-type H. ducreyi znuA gene was also able to complement a H. influenzae pzp1 mutation. The H. ducreyi znuA isogenic mutant exhibited significantly decreased virulence (P = 0.0001) when tested in the temperature-dependent rabbit model for experimental chancroid. This decreased virulence was not observed when the znuA mutant was complemented with the wild-type H. ducreyi znuA gene provided in trans.

Original languageEnglish (US)
Pages (from-to)5060-5068
Number of pages9
JournalInfection and Immunity
Volume67
Issue number10
StatePublished - 1999

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Haemophilus ducreyi
Zinc
Carrier Proteins
Genes
Virulence
Proteins
Chancroid
Periplasm
Mutation
Insertional Mutagenesis
Escherichia coli Proteins
Human Influenza
Cations
Theoretical Models

ASJC Scopus subject areas

  • Immunology

Cite this

Lewis, D. A., Klesney-Tait, J., Lumbley, S. R., Ward, C. K., Latimer, J. L., Ison, C. A., & Hansen, E. J. (1999). Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi. Infection and Immunity, 67(10), 5060-5068.

Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi. / Lewis, David A.; Klesney-Tait, Julia; Lumbley, Sheryl R.; Ward, Christine K.; Latimer, Jo L.; Ison, Catherine A.; Hansen, Eric J.

In: Infection and Immunity, Vol. 67, No. 10, 1999, p. 5060-5068.

Research output: Contribution to journalArticle

Lewis, DA, Klesney-Tait, J, Lumbley, SR, Ward, CK, Latimer, JL, Ison, CA & Hansen, EJ 1999, 'Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi', Infection and Immunity, vol. 67, no. 10, pp. 5060-5068.
Lewis DA, Klesney-Tait J, Lumbley SR, Ward CK, Latimer JL, Ison CA et al. Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi. Infection and Immunity. 1999;67(10):5060-5068.
Lewis, David A. ; Klesney-Tait, Julia ; Lumbley, Sheryl R. ; Ward, Christine K. ; Latimer, Jo L. ; Ison, Catherine A. ; Hansen, Eric J. / Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi. In: Infection and Immunity. 1999 ; Vol. 67, No. 10. pp. 5060-5068.
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