Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi

David A. Lewis, Julia Klesney-Tait, Sheryl R. Lumbley, Christine K. Ward, Jo L. Latimer, Catherine A. Ison, Eric J. Hansen

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

The znuA gene of Haemophilus ducreyi encodes a 32-kDa (mature) protein that has homology to both the ZnuA protein of Escherichia coli and the Pzp1 protein of H. infiuenzae; both of these latter proteins are members of a growing family of prokaryotic zinc transporters. Inactivation of the H. ducreyi 35000 znuA gene by insertional mutagenesis resulted in a mutant that grew more slowly than the wild-type parent strain in vitro unless ZnCl2 was provided at a final concentration of 100 μM. Other cations tested did not restore growth of this H. ducreyi mutant to wild-type levels. The H. ducreyi ZnuA protein was localized to the periplasm, where it is believed to function as the binding component of a zinc transport system. Complementation of the znuA mutation with the wild-type H. ducreyi znuA gene provided in trans restored the ability of this H. ducreyi mutant to grow normally in the absence of exogenously added ZnCl2. The wild-type H. ducreyi znuA gene was also able to complement a H. influenzae pzp1 mutation. The H. ducreyi znuA isogenic mutant exhibited significantly decreased virulence (P = 0.0001) when tested in the temperature-dependent rabbit model for experimental chancroid. This decreased virulence was not observed when the znuA mutant was complemented with the wild-type H. ducreyi znuA gene provided in trans.

Original languageEnglish (US)
Pages (from-to)5060-5068
Number of pages9
JournalInfection and immunity
Volume67
Issue number10
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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    Lewis, D. A., Klesney-Tait, J., Lumbley, S. R., Ward, C. K., Latimer, J. L., Ison, C. A., & Hansen, E. J. (1999). Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi. Infection and immunity, 67(10), 5060-5068. https://doi.org/10.1128/iai.67.10.5060-5068.1999