Identification of three distinct tumor suppressor loci on the short arm of chromosome 9 in small cell lung cancer

Se K. Kim, Jae Y. Ro, Bonnie L. Kemp, Jin Soo Lee, Tae Jung Kwon, Kwun M. Fong, Yoshitaka Sekido, John D. Minna, Waun K. Hong, Li Mao

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Abstract

Deletion at 9p21 is frequent in many tumor types. A candidate tumor suppressor gene, p16(INK4a), was mapped to this region and is frequently inactivated by several different mechanism in many tumor types, including non-small cell lung cancer, but not in small cell lung cancer (SCLC). p16 functions as a cyclin/CDK inhibitor to prevent phosphorylation of pRB. It has been demonstrated that most SCLCs have lost pRB but retained p16, and the inactivation of pRB excludes the inactivation of p16 and vice versa. To determine the potential existence of other tumor suppressor genes on the short arm of chromosome 9 in SCLC, we tested 46 primary SCLCs by microsatellite analysis. We found that more than 89% of the tumors exhibited loss of heterozygosity (LOH) at 9p with three distinct minimal deleted areas. Among those areas, LOH at 9p21 was most frequent (86%), with a peak at a marker 150 kb telomeric to p16(INK4a). LOH was also observed in more than 50% of the tumors at two other regions, 9p22 and 9p13. Our data strongly suggest the presence of at least three novel tumor suppressor loci on 9p in SCLC, and further investigations to clone candidate tumor suppressor genes are warranted.

Original languageEnglish (US)
Pages (from-to)400-403
Number of pages4
JournalCancer Research
Volume57
Issue number3
StatePublished - 1997

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Chromosomes, Human, Pair 9
Small Cell Lung Carcinoma
Loss of Heterozygosity
Tumor Suppressor Genes
Neoplasms
Cyclins
Non-Small Cell Lung Carcinoma
Microsatellite Repeats
Clone Cells
Phosphorylation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kim, S. K., Ro, J. Y., Kemp, B. L., Lee, J. S., Kwon, T. J., Fong, K. M., ... Mao, L. (1997). Identification of three distinct tumor suppressor loci on the short arm of chromosome 9 in small cell lung cancer. Cancer Research, 57(3), 400-403.

Identification of three distinct tumor suppressor loci on the short arm of chromosome 9 in small cell lung cancer. / Kim, Se K.; Ro, Jae Y.; Kemp, Bonnie L.; Lee, Jin Soo; Kwon, Tae Jung; Fong, Kwun M.; Sekido, Yoshitaka; Minna, John D.; Hong, Waun K.; Mao, Li.

In: Cancer Research, Vol. 57, No. 3, 1997, p. 400-403.

Research output: Contribution to journalArticle

Kim, SK, Ro, JY, Kemp, BL, Lee, JS, Kwon, TJ, Fong, KM, Sekido, Y, Minna, JD, Hong, WK & Mao, L 1997, 'Identification of three distinct tumor suppressor loci on the short arm of chromosome 9 in small cell lung cancer', Cancer Research, vol. 57, no. 3, pp. 400-403.
Kim, Se K. ; Ro, Jae Y. ; Kemp, Bonnie L. ; Lee, Jin Soo ; Kwon, Tae Jung ; Fong, Kwun M. ; Sekido, Yoshitaka ; Minna, John D. ; Hong, Waun K. ; Mao, Li. / Identification of three distinct tumor suppressor loci on the short arm of chromosome 9 in small cell lung cancer. In: Cancer Research. 1997 ; Vol. 57, No. 3. pp. 400-403.
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