IFN-γ-dependent IL-7 gene regulation in keratinocytes

K. Ariizumi, Y. Meng, P. R. Bergstresser, A. Takashima

Research output: Contribution to journalArticle

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Abstract

IL-7 plays a central role in regulating the growth and differentiation of T cells. We have reported previously that epidermal keratinocytes produce biologically relevant amounts of IL-7, thereby supporting the growth of epidermal γδ T cells. In this report, we report that IL-7 gene expression is regulated in keratinocytes by IFN-γ. Treatment of Pam 212 keratinocytes with IFN-γ induced a preferential expression of 2.6- and 1.5-kb IL-7 mRNAs, in addition to the 2.9- and 1.7-kb mRNAs that are expressed constitutively. The 2.6- and 1.5-kb mRNAs are produced through the use of alternative transcription initiation sites; these mRNAs are transcribed within 250 bp from the coding sequence, whereas 2.9- and 1.7-kb mRNAs contain >400 bases in the 5'-untranslated region. IFN-γ appears to promote this conversion through the IFN-stimulated response element (ISRE), which is located 270 bp upstream from the coding sequence. ISRE is followed by the initiator, a non-TATA-type transcription control element. Functional relevance of the ISRE/initiator complex was suggested by the observations that IFN-γ-dependent transcription was initiated from immediately downstream of this complex, and that its deletion resulted in an abrogated IFN-γ responsiveness in transcriptional regulation. These results document a novel mechanism by which IL-7 gene expression is regulated in keratinocytes by a cytokine produced by T cells (IFN-γ).

Original languageEnglish (US)
Pages (from-to)6031-6039
Number of pages9
JournalJournal of Immunology
Volume154
Issue number11
StatePublished - 1995

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Interleukin-7
Keratinocytes
Response Elements
Messenger RNA
Genes
T-Lymphocytes
Gene Expression
5' Untranslated Regions
Transcription Initiation Site
Growth
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Ariizumi, K., Meng, Y., Bergstresser, P. R., & Takashima, A. (1995). IFN-γ-dependent IL-7 gene regulation in keratinocytes. Journal of Immunology, 154(11), 6031-6039.

IFN-γ-dependent IL-7 gene regulation in keratinocytes. / Ariizumi, K.; Meng, Y.; Bergstresser, P. R.; Takashima, A.

In: Journal of Immunology, Vol. 154, No. 11, 1995, p. 6031-6039.

Research output: Contribution to journalArticle

Ariizumi, K, Meng, Y, Bergstresser, PR & Takashima, A 1995, 'IFN-γ-dependent IL-7 gene regulation in keratinocytes', Journal of Immunology, vol. 154, no. 11, pp. 6031-6039.
Ariizumi K, Meng Y, Bergstresser PR, Takashima A. IFN-γ-dependent IL-7 gene regulation in keratinocytes. Journal of Immunology. 1995;154(11):6031-6039.
Ariizumi, K. ; Meng, Y. ; Bergstresser, P. R. ; Takashima, A. / IFN-γ-dependent IL-7 gene regulation in keratinocytes. In: Journal of Immunology. 1995 ; Vol. 154, No. 11. pp. 6031-6039.
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