Abstract
IFN-γ is a pleiotropic lymphokine that influences the isotypes of immunoglobulin secreted by B cells. IFN-γ inhibits the secretion of IgG3, IgG2b, IgG1, and IgE, and enhances the secretion of IgG2a. We have examined the mechanism of IFN-γ-mediated enhancement of IgG2a secretion in sorted populations of B cells and find that IFN-γ reproducibly stimulates a twofold increase in the precursor frequency of IgG2a-secreting cells in the sIgG2a+ population. Additionally, we find that IFN-γ does not induce an increase in the clone size of IgG2a-secreting cells. IFN-γ stimulates a twofold increase in the precursor frequency of IgG2a-secreting cells from sIgG- and unsorted B cells which can be attributed to an increase in IgG2a secretion from IgG2a-committed cells in these populations. Hence, under the culture conditions utilized in these studies, IFN-γ enhances IgG2a secretion from IgG2a-committed cells and does not induce a class switch.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-104 |
| Number of pages | 10 |
| Journal | Cellular Immunology |
| Volume | 135 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1991 |
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ASJC Scopus subject areas
- Cell Biology
- Immunology
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IFN-γ enhances secretion of IgG2a from IgG2a-committed LPS-stimulated murine B cells : Implications for the role of IFN-γ in class switching. / Bossie, Alexis; Vitetta, Ellen S.
In: Cellular Immunology, Vol. 135, No. 1, 1991, p. 95-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - IFN-γ enhances secretion of IgG2a from IgG2a-committed LPS-stimulated murine B cells
T2 - Implications for the role of IFN-γ in class switching
AU - Bossie, Alexis
AU - Vitetta, Ellen S.
PY - 1991
Y1 - 1991
N2 - IFN-γ is a pleiotropic lymphokine that influences the isotypes of immunoglobulin secreted by B cells. IFN-γ inhibits the secretion of IgG3, IgG2b, IgG1, and IgE, and enhances the secretion of IgG2a. We have examined the mechanism of IFN-γ-mediated enhancement of IgG2a secretion in sorted populations of B cells and find that IFN-γ reproducibly stimulates a twofold increase in the precursor frequency of IgG2a-secreting cells in the sIgG2a+ population. Additionally, we find that IFN-γ does not induce an increase in the clone size of IgG2a-secreting cells. IFN-γ stimulates a twofold increase in the precursor frequency of IgG2a-secreting cells from sIgG- and unsorted B cells which can be attributed to an increase in IgG2a secretion from IgG2a-committed cells in these populations. Hence, under the culture conditions utilized in these studies, IFN-γ enhances IgG2a secretion from IgG2a-committed cells and does not induce a class switch.
AB - IFN-γ is a pleiotropic lymphokine that influences the isotypes of immunoglobulin secreted by B cells. IFN-γ inhibits the secretion of IgG3, IgG2b, IgG1, and IgE, and enhances the secretion of IgG2a. We have examined the mechanism of IFN-γ-mediated enhancement of IgG2a secretion in sorted populations of B cells and find that IFN-γ reproducibly stimulates a twofold increase in the precursor frequency of IgG2a-secreting cells in the sIgG2a+ population. Additionally, we find that IFN-γ does not induce an increase in the clone size of IgG2a-secreting cells. IFN-γ stimulates a twofold increase in the precursor frequency of IgG2a-secreting cells from sIgG- and unsorted B cells which can be attributed to an increase in IgG2a secretion from IgG2a-committed cells in these populations. Hence, under the culture conditions utilized in these studies, IFN-γ enhances IgG2a secretion from IgG2a-committed cells and does not induce a class switch.
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U2 - 10.1016/0008-8749(91)90257-C
DO - 10.1016/0008-8749(91)90257-C
M3 - Article
C2 - 1902147
AN - SCOPUS:0025811173
VL - 135
SP - 95
EP - 104
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -