IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure

Lenette L. Lu, Malisa T. Smith, Krystle K.Q. Yu, Corinne Luedemann, Todd J. Suscovich, Patricia S. Grace, Adam Cain, Wen Han Yu, Tanya R. McKitrick, Douglas Lauffenburger, Richard D. Cummings, Harriet Mayanja-Kizza, Thomas R. Hawn, W. Henry Boom, Catherine M. Stein, Sarah M. Fortune, Chetan Seshadri, Galit Alter

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Exposure to Mycobacterium tuberculosis (Mtb) results in heterogeneous clinical outcomes including primary progressive tuberculosis and latent Mtb infection (LTBI). Mtb infection is identified using the tuberculin skin test and interferon-γ (IFN-γ) release assay IGRA, and a positive result may prompt chemoprophylaxis to prevent progression to tuberculosis. In the present study, we report on a cohort of Ugandan individuals who were household contacts of patients with TB. These individuals were highly exposed to Mtb but tested negative disease by IFN-γ release assay and tuberculin skin test, ‘resisting’ development of classic LTBI. We show that ‘resisters’ possess IgM, class-switched IgG antibody responses and non-IFN-γ T cell responses to the Mtb-specific proteins ESAT6 and CFP10, immunologic evidence of exposure to Mtb. Compared to subjects with classic LTBI, ‘resisters’ display enhanced antibody avidity and distinct Mtb-specific IgG Fc profiles. These data reveal a distinctive adaptive immune profile among Mtb-exposed subjects, supporting an expanded definition of the host response to Mtb exposure, with implications for public health and the design of clinical trials.

Original languageEnglish (US)
Pages (from-to)977-987
Number of pages11
JournalNature Medicine
Volume25
Issue number6
DOIs
StatePublished - Jun 1 2019
Externally publishedYes

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Tuberculin
Mycobacterium tuberculosis
Interferons
Assays
Skin
Immunoglobulin G
Biomarkers
T-cells
Antibodies
Public health
Immunoglobulin M
Tuberculin Test
Mycobacterium Infections
Skin Tests
Tuberculosis
Proteins
Latent Tuberculosis
Antibody Affinity
Chemoprevention
Infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lu, L. L., Smith, M. T., Yu, K. K. Q., Luedemann, C., Suscovich, T. J., Grace, P. S., ... Alter, G. (2019). IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure. Nature Medicine, 25(6), 977-987. https://doi.org/10.1038/s41591-019-0441-3

IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure. / Lu, Lenette L.; Smith, Malisa T.; Yu, Krystle K.Q.; Luedemann, Corinne; Suscovich, Todd J.; Grace, Patricia S.; Cain, Adam; Yu, Wen Han; McKitrick, Tanya R.; Lauffenburger, Douglas; Cummings, Richard D.; Mayanja-Kizza, Harriet; Hawn, Thomas R.; Boom, W. Henry; Stein, Catherine M.; Fortune, Sarah M.; Seshadri, Chetan; Alter, Galit.

In: Nature Medicine, Vol. 25, No. 6, 01.06.2019, p. 977-987.

Research output: Contribution to journalArticle

Lu, LL, Smith, MT, Yu, KKQ, Luedemann, C, Suscovich, TJ, Grace, PS, Cain, A, Yu, WH, McKitrick, TR, Lauffenburger, D, Cummings, RD, Mayanja-Kizza, H, Hawn, TR, Boom, WH, Stein, CM, Fortune, SM, Seshadri, C & Alter, G 2019, 'IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure', Nature Medicine, vol. 25, no. 6, pp. 977-987. https://doi.org/10.1038/s41591-019-0441-3
Lu LL, Smith MT, Yu KKQ, Luedemann C, Suscovich TJ, Grace PS et al. IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure. Nature Medicine. 2019 Jun 1;25(6):977-987. https://doi.org/10.1038/s41591-019-0441-3
Lu, Lenette L. ; Smith, Malisa T. ; Yu, Krystle K.Q. ; Luedemann, Corinne ; Suscovich, Todd J. ; Grace, Patricia S. ; Cain, Adam ; Yu, Wen Han ; McKitrick, Tanya R. ; Lauffenburger, Douglas ; Cummings, Richard D. ; Mayanja-Kizza, Harriet ; Hawn, Thomas R. ; Boom, W. Henry ; Stein, Catherine M. ; Fortune, Sarah M. ; Seshadri, Chetan ; Alter, Galit. / IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure. In: Nature Medicine. 2019 ; Vol. 25, No. 6. pp. 977-987.
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