IgE-challenged human lung mast cells excite vagal sensory neurons in vitro

R. Greene, J. Fowler, D. MacGlashan, D. Weinreich

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Substances released from immunoglobin (Ig) E-stimulated mast cells are likely to be among the chemical mediators responsible for changes in the vagal component of airway reactivity. We have attempted to identify a direct role for mast cell mediators in the control of visceral afferent excitability by examining intracellular electrophysiological changes in vagal neurons after application of extracts prepared from purified and IgE-stimulated human lung mast cells (HLMC). HLMC's, applied by superfusion or by focal pressure ejection from micropipettes, reversibly enhanced the excitability of a subpopulation of rabbit visceral sensory C-fiber neurons by 1) abolishing the slow Ca2+-dependent post-spike afterhyperpolarization that uniquely resides in these neurons and controls their spike frequency, 2) depolarizing the cell membrane potential, and 3) increasing membrane input resistance. Control HLMC prepared by subjecting purified human lung mast cells to normal goat serum had no measurable affects on neuronal excitability. The immunologically released constituents responsible for these excitability changes are likely to be lipid mediators, because essentially all biological activity is extractable into an organic phase after methanol-chloroform solvent extraction of the HLMC preparations. These results provide the first unambiguous evidence that products released from immunologically challenged HLMC's directly affect visceral afferent nerve cell function.

Original languageEnglish (US)
Pages (from-to)2249-2253
Number of pages5
JournalJournal of Applied Physiology
Volume64
Issue number5
StatePublished - 1988

Fingerprint

Sensory Receptor Cells
Mast Cells
Immunoglobulin E
Lung
Visceral Afferents
Neurons
Unmyelinated Nerve Fibers
Chloroform
Goats
Membrane Potentials
Methanol
In Vitro Techniques
Cell Membrane
Rabbits
Lipids
Pressure
Membranes
Serum

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Greene, R., Fowler, J., MacGlashan, D., & Weinreich, D. (1988). IgE-challenged human lung mast cells excite vagal sensory neurons in vitro. Journal of Applied Physiology, 64(5), 2249-2253.

IgE-challenged human lung mast cells excite vagal sensory neurons in vitro. / Greene, R.; Fowler, J.; MacGlashan, D.; Weinreich, D.

In: Journal of Applied Physiology, Vol. 64, No. 5, 1988, p. 2249-2253.

Research output: Contribution to journalArticle

Greene, R, Fowler, J, MacGlashan, D & Weinreich, D 1988, 'IgE-challenged human lung mast cells excite vagal sensory neurons in vitro', Journal of Applied Physiology, vol. 64, no. 5, pp. 2249-2253.
Greene, R. ; Fowler, J. ; MacGlashan, D. ; Weinreich, D. / IgE-challenged human lung mast cells excite vagal sensory neurons in vitro. In: Journal of Applied Physiology. 1988 ; Vol. 64, No. 5. pp. 2249-2253.
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