IgG and IgM autoantibody differences in discoid and systemic lupus patients

Benjamin F. Chong, Lin Chiang Tseng, Thomas Lee, Rebecca Vasquez, Quan Z. Li, Song Zhang, David R. Karp, Nancy J. Olsen, Chandra Mohan

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Systemic lupus erythematosus (SLE) patients with discoid lupus erythematosus (DLE) were reported to have milder disease. To test this observation, we used sandwich arrays containing 98 autoantigens to compare autoantibody profiles of SLE subjects without DLE (DLE-SLE\+) (N = 9), SLE subjects with DLE (DLE\+SLE+) (N = 10), DLE subjects without SLE (DLE+SLE-) (N = 11), and healthy controls (N = 11). We validated differentially expressed autoantibodies using immunoassays in DLE-SLE+ (N = 18), DLE+SLE+ (N = 17), DLE+SLE-(N = 23), and healthy subjects (N = 22). Arrays showed 15 IgG autoantibodies (10 against nuclear antigens) and 4 IgM autoantibodies that were differentially expressed (q-value < 0.05). DLE-SLE+ subjects had higher IgG autoantibodies against double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), doublestranded RNA (dsRNA), histone H2A and H2B, and SS-A (52 kDa) compared with all other groups including DLE+SLE+ subjects (P<0.05). Immunoassays measuring anti-dsDNA,-ssDNA, and-SS-A (52 kDa) IgG autoantibodies showed similar trends (P<0.05). Healthy and DLE+SLE-subjects expressed higher IgM autoantibodies against alpha beta crystallin, lipopolysaccharide, heat-shock cognate 70, and desmoglein-3 compared with DLE+SLE+ and DLE-SLE+ subjects. IgG:IgM ratios of autoantibodies against nuclear antigens progressively rose from healthy to DLE-SLE+ subjects. In conclusion, lower IgG autoantibodies against nuclear antigens in DLE+SLE+ versus DLE-SLE+ subjects suggest that DLE indicates lower disease severity. Higher IgM autoantibodies against selected antigens in healthy and DLE+SLE-subjects may be nonpathogenic.

Original languageEnglish (US)
Pages (from-to)2770-2779
Number of pages10
JournalJournal of Investigative Dermatology
Volume132
Issue number12
DOIs
StatePublished - Dec 2012

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Discoid Lupus Erythematosus
Autoantibodies
Systemic Lupus Erythematosus
Immunoglobulin M
Immunoglobulin G
Nuclear Antigens
Desmoglein 3
beta-Crystallins
Single-Stranded DNA
Immunoassay
Histones
Lipopolysaccharides

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

IgG and IgM autoantibody differences in discoid and systemic lupus patients. / Chong, Benjamin F.; Tseng, Lin Chiang; Lee, Thomas; Vasquez, Rebecca; Li, Quan Z.; Zhang, Song; Karp, David R.; Olsen, Nancy J.; Mohan, Chandra.

In: Journal of Investigative Dermatology, Vol. 132, No. 12, 12.2012, p. 2770-2779.

Research output: Contribution to journalArticle

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abstract = "Systemic lupus erythematosus (SLE) patients with discoid lupus erythematosus (DLE) were reported to have milder disease. To test this observation, we used sandwich arrays containing 98 autoantigens to compare autoantibody profiles of SLE subjects without DLE (DLE-SLE\+) (N = 9), SLE subjects with DLE (DLE\+SLE+) (N = 10), DLE subjects without SLE (DLE+SLE-) (N = 11), and healthy controls (N = 11). We validated differentially expressed autoantibodies using immunoassays in DLE-SLE+ (N = 18), DLE+SLE+ (N = 17), DLE+SLE-(N = 23), and healthy subjects (N = 22). Arrays showed 15 IgG autoantibodies (10 against nuclear antigens) and 4 IgM autoantibodies that were differentially expressed (q-value < 0.05). DLE-SLE+ subjects had higher IgG autoantibodies against double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), doublestranded RNA (dsRNA), histone H2A and H2B, and SS-A (52 kDa) compared with all other groups including DLE+SLE+ subjects (P<0.05). Immunoassays measuring anti-dsDNA,-ssDNA, and-SS-A (52 kDa) IgG autoantibodies showed similar trends (P<0.05). Healthy and DLE+SLE-subjects expressed higher IgM autoantibodies against alpha beta crystallin, lipopolysaccharide, heat-shock cognate 70, and desmoglein-3 compared with DLE+SLE+ and DLE-SLE+ subjects. IgG:IgM ratios of autoantibodies against nuclear antigens progressively rose from healthy to DLE-SLE+ subjects. In conclusion, lower IgG autoantibodies against nuclear antigens in DLE+SLE+ versus DLE-SLE+ subjects suggest that DLE indicates lower disease severity. Higher IgM autoantibodies against selected antigens in healthy and DLE+SLE-subjects may be nonpathogenic.",
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T1 - IgG and IgM autoantibody differences in discoid and systemic lupus patients

AU - Chong, Benjamin F.

AU - Tseng, Lin Chiang

AU - Lee, Thomas

AU - Vasquez, Rebecca

AU - Li, Quan Z.

AU - Zhang, Song

AU - Karp, David R.

AU - Olsen, Nancy J.

AU - Mohan, Chandra

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N2 - Systemic lupus erythematosus (SLE) patients with discoid lupus erythematosus (DLE) were reported to have milder disease. To test this observation, we used sandwich arrays containing 98 autoantigens to compare autoantibody profiles of SLE subjects without DLE (DLE-SLE\+) (N = 9), SLE subjects with DLE (DLE\+SLE+) (N = 10), DLE subjects without SLE (DLE+SLE-) (N = 11), and healthy controls (N = 11). We validated differentially expressed autoantibodies using immunoassays in DLE-SLE+ (N = 18), DLE+SLE+ (N = 17), DLE+SLE-(N = 23), and healthy subjects (N = 22). Arrays showed 15 IgG autoantibodies (10 against nuclear antigens) and 4 IgM autoantibodies that were differentially expressed (q-value < 0.05). DLE-SLE+ subjects had higher IgG autoantibodies against double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), doublestranded RNA (dsRNA), histone H2A and H2B, and SS-A (52 kDa) compared with all other groups including DLE+SLE+ subjects (P<0.05). Immunoassays measuring anti-dsDNA,-ssDNA, and-SS-A (52 kDa) IgG autoantibodies showed similar trends (P<0.05). Healthy and DLE+SLE-subjects expressed higher IgM autoantibodies against alpha beta crystallin, lipopolysaccharide, heat-shock cognate 70, and desmoglein-3 compared with DLE+SLE+ and DLE-SLE+ subjects. IgG:IgM ratios of autoantibodies against nuclear antigens progressively rose from healthy to DLE-SLE+ subjects. In conclusion, lower IgG autoantibodies against nuclear antigens in DLE+SLE+ versus DLE-SLE+ subjects suggest that DLE indicates lower disease severity. Higher IgM autoantibodies against selected antigens in healthy and DLE+SLE-subjects may be nonpathogenic.

AB - Systemic lupus erythematosus (SLE) patients with discoid lupus erythematosus (DLE) were reported to have milder disease. To test this observation, we used sandwich arrays containing 98 autoantigens to compare autoantibody profiles of SLE subjects without DLE (DLE-SLE\+) (N = 9), SLE subjects with DLE (DLE\+SLE+) (N = 10), DLE subjects without SLE (DLE+SLE-) (N = 11), and healthy controls (N = 11). We validated differentially expressed autoantibodies using immunoassays in DLE-SLE+ (N = 18), DLE+SLE+ (N = 17), DLE+SLE-(N = 23), and healthy subjects (N = 22). Arrays showed 15 IgG autoantibodies (10 against nuclear antigens) and 4 IgM autoantibodies that were differentially expressed (q-value < 0.05). DLE-SLE+ subjects had higher IgG autoantibodies against double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), doublestranded RNA (dsRNA), histone H2A and H2B, and SS-A (52 kDa) compared with all other groups including DLE+SLE+ subjects (P<0.05). Immunoassays measuring anti-dsDNA,-ssDNA, and-SS-A (52 kDa) IgG autoantibodies showed similar trends (P<0.05). Healthy and DLE+SLE-subjects expressed higher IgM autoantibodies against alpha beta crystallin, lipopolysaccharide, heat-shock cognate 70, and desmoglein-3 compared with DLE+SLE+ and DLE-SLE+ subjects. IgG:IgM ratios of autoantibodies against nuclear antigens progressively rose from healthy to DLE-SLE+ subjects. In conclusion, lower IgG autoantibodies against nuclear antigens in DLE+SLE+ versus DLE-SLE+ subjects suggest that DLE indicates lower disease severity. Higher IgM autoantibodies against selected antigens in healthy and DLE+SLE-subjects may be nonpathogenic.

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