IIV-6 Inhibits NF-κB Responses in Drosophila

Cara West, Florentina Rus, Ying Chen, Anni Kleino, Monique Gangloff, Don Brad Gammon, Neal Silverman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The host immune response and virus-encoded immune evasion proteins pose constant, mutual selective pressure on each other. Virally encoded immune evasion proteins also indicate which host pathways must be inhibited to allow for viral replication. Here, we show that IIV-6 is capable of inhibiting the two Drosophila NF-κB signaling pathways, Imd and Toll. Antimicrobial peptide (AMP) gene induction downstream of either pathway is suppressed when cells infected with IIV-6 are also stimulated with Toll or Imd ligands. We find that cleavage of both Imd and Relish, as well as Relish nuclear translocation, three key points in Imd signal transduction, occur in IIV-6 infected cells, indicating that the mechanism of viral inhibition is farther downstream, at the level of Relish promoter binding or transcriptional activation. Additionally, flies co-infected with both IIV-6 and the Gram-negative bacterium, Erwinia carotovora carotovora, succumb to infection more rapidly than flies singly infected with either the virus or the bacterium. These findings demonstrate how pre-existing infections can have a dramatic and negative effect on secondary infections, and establish a Drosophila model to study confection susceptibility.

Original languageEnglish (US)
JournalViruses
Volume11
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Immune Evasion
Diptera
Drosophila
Pectobacterium carotovorum
Candy
Viruses
Infection
Gram-Negative Bacteria
Coinfection
Transcriptional Activation
Signal Transduction
Proteins
Ligands
Bacteria
Peptides
Genes

Keywords

  • DNA virus
  • host-pathogen interactions
  • IIV-6
  • Imd
  • immunomodulators
  • NF-κB
  • viral immune evasion

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

West, C., Rus, F., Chen, Y., Kleino, A., Gangloff, M., Gammon, D. B., & Silverman, N. (2019). IIV-6 Inhibits NF-κB Responses in Drosophila. Viruses, 11(5). https://doi.org/10.3390/v11050409

IIV-6 Inhibits NF-κB Responses in Drosophila. / West, Cara; Rus, Florentina; Chen, Ying; Kleino, Anni; Gangloff, Monique; Gammon, Don Brad; Silverman, Neal.

In: Viruses, Vol. 11, No. 5, 01.05.2019.

Research output: Contribution to journalArticle

West, C, Rus, F, Chen, Y, Kleino, A, Gangloff, M, Gammon, DB & Silverman, N 2019, 'IIV-6 Inhibits NF-κB Responses in Drosophila', Viruses, vol. 11, no. 5. https://doi.org/10.3390/v11050409
West C, Rus F, Chen Y, Kleino A, Gangloff M, Gammon DB et al. IIV-6 Inhibits NF-κB Responses in Drosophila. Viruses. 2019 May 1;11(5). https://doi.org/10.3390/v11050409
West, Cara ; Rus, Florentina ; Chen, Ying ; Kleino, Anni ; Gangloff, Monique ; Gammon, Don Brad ; Silverman, Neal. / IIV-6 Inhibits NF-κB Responses in Drosophila. In: Viruses. 2019 ; Vol. 11, No. 5.
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