IIV-6 inhibits NF-ΚB responses in drosophila

Cara West, Florentina Rus, Ying Chen, Anni Kleino, Monique Gangloff, Don B. Gammon, Neal Silverman

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The host immune response and virus-encoded immune evasion proteins pose constant, mutual selective pressure on each other. Virally encoded immune evasion proteins also indicate which host pathways must be inhibited to allow for viral replication. Here, we show that IIV-6 is capable of inhibiting the two Drosophila NF-?B signaling pathways, Imd and Toll. Antimicrobial peptide (AMP) gene induction downstream of either pathway is suppressed when cells infected with IIV-6 are also stimulated with Toll or Imd ligands. We find that cleavage of both Imd and Relish, as well as Relish nuclear translocation, three key points in Imd signal transduction, occur in IIV-6 infected cells, indicating that the mechanism of viral inhibition is farther downstream, at the level of Relish promoter binding or transcriptional activation. Additionally, flies co-infected with both IIV-6 and the Gram-negative bacterium, Erwinia carotovora carotovora, succumb to infection more rapidly than flies singly infected with either the virus or the bacterium. These findings demonstrate how pre-existing infections can have a dramatic and negative effect on secondary infections, and establish a Drosophila model to study confection susceptibility.

Original languageEnglish (US)
Article number409
JournalViruses
Volume11
Issue number5
DOIs
StatePublished - May 2019

Keywords

  • DNA virus
  • Host-pathogen interactions
  • IIV-6
  • Imd
  • Immunomodulators
  • NF-ΚB
  • Viral immune evasion

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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