IL-10 polymorphism associated with decreased risk for mortality after burn injury

Ryan M. Huebinger, Fernando Rivera-Chavez, Ling Yu Chang, Ming Mei Liu, Joseph P. Minei, Gary F. Purdue, John L. Hunt, Brett D. Arnoldo, Robert C. Barber

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: Evaluation of single nucleotide polymorphisms (SNPs) in the interleukin10 promoter (592 and 819) on risk for death after burn injury. Methods: Association between the IL-10 SNPs and outcome after burn injury was evaluated in a cohort of 265 patients from Parkland Hospital, Dallas, TX with ≥15% TBSA burns without non-burn trauma (ISS ≤ 16), traumatic or anoxic brain injury or spinal cord injury, who survived >48 h under an IRB-approved protocol. Clinical data were collected prospectively and genotyping was conducted by TaqMan assay. Whole blood from 31 healthy volunteers was stimulated with LPS (100 ng/mL) to determine the level of IL-10 expression for each allele by enzyme-linked immunosorbent assay (ELISA). Results: After adjustment for percent total body surface area (TBSA) burned, inhalation injury, age, gender, and race/ethnicity, carriage of 592A and/or 819T was significantly associated (P = 0.014) with a decreased risk for death (adjusted odds ratio: 0.404; 95% CI: 0.197-0.829). As the candidate SNPs were in complete linkage disequilibrium, it was not possible to distinguish which allele was associated with decreased mortality risk. Age, inhalation injury, and full-thickness burn size were significantly associated with increased risk for death. In the LPS stimulated blood of healthy controls, carriage of the -592A and/or -819T allele demonstrated a trend for decreased levels of IL-10 (P = 0.079). Conclusion: Carriage of the 592A and/or 819T allele in the IL-10 promoter appears to reduce the risk for death after burn injury.

Original languageEnglish (US)
JournalJournal of Surgical Research
Volume164
Issue number1
DOIs
StatePublished - Nov 2010

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Interleukin-10
Mortality
Wounds and Injuries
Alleles
Single Nucleotide Polymorphism
Body Surface Area
Inhalation
Research Ethics Committees
Linkage Disequilibrium
Spinal Cord Injuries
Burns
Brain Injuries
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Odds Ratio

Keywords

  • burn injury
  • genetic association
  • interleukin-10
  • mortality

ASJC Scopus subject areas

  • Surgery

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IL-10 polymorphism associated with decreased risk for mortality after burn injury. / Huebinger, Ryan M.; Rivera-Chavez, Fernando; Chang, Ling Yu; Liu, Ming Mei; Minei, Joseph P.; Purdue, Gary F.; Hunt, John L.; Arnoldo, Brett D.; Barber, Robert C.

In: Journal of Surgical Research, Vol. 164, No. 1, 11.2010.

Research output: Contribution to journalArticle

Huebinger, Ryan M. ; Rivera-Chavez, Fernando ; Chang, Ling Yu ; Liu, Ming Mei ; Minei, Joseph P. ; Purdue, Gary F. ; Hunt, John L. ; Arnoldo, Brett D. ; Barber, Robert C. / IL-10 polymorphism associated with decreased risk for mortality after burn injury. In: Journal of Surgical Research. 2010 ; Vol. 164, No. 1.
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abstract = "Objective: Evaluation of single nucleotide polymorphisms (SNPs) in the interleukin10 promoter (592 and 819) on risk for death after burn injury. Methods: Association between the IL-10 SNPs and outcome after burn injury was evaluated in a cohort of 265 patients from Parkland Hospital, Dallas, TX with ≥15{\%} TBSA burns without non-burn trauma (ISS ≤ 16), traumatic or anoxic brain injury or spinal cord injury, who survived >48 h under an IRB-approved protocol. Clinical data were collected prospectively and genotyping was conducted by TaqMan assay. Whole blood from 31 healthy volunteers was stimulated with LPS (100 ng/mL) to determine the level of IL-10 expression for each allele by enzyme-linked immunosorbent assay (ELISA). Results: After adjustment for percent total body surface area (TBSA) burned, inhalation injury, age, gender, and race/ethnicity, carriage of 592A and/or 819T was significantly associated (P = 0.014) with a decreased risk for death (adjusted odds ratio: 0.404; 95{\%} CI: 0.197-0.829). As the candidate SNPs were in complete linkage disequilibrium, it was not possible to distinguish which allele was associated with decreased mortality risk. Age, inhalation injury, and full-thickness burn size were significantly associated with increased risk for death. In the LPS stimulated blood of healthy controls, carriage of the -592A and/or -819T allele demonstrated a trend for decreased levels of IL-10 (P = 0.079). Conclusion: Carriage of the 592A and/or 819T allele in the IL-10 promoter appears to reduce the risk for death after burn injury.",
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AU - Minei, Joseph P.

AU - Purdue, Gary F.

AU - Hunt, John L.

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N2 - Objective: Evaluation of single nucleotide polymorphisms (SNPs) in the interleukin10 promoter (592 and 819) on risk for death after burn injury. Methods: Association between the IL-10 SNPs and outcome after burn injury was evaluated in a cohort of 265 patients from Parkland Hospital, Dallas, TX with ≥15% TBSA burns without non-burn trauma (ISS ≤ 16), traumatic or anoxic brain injury or spinal cord injury, who survived >48 h under an IRB-approved protocol. Clinical data were collected prospectively and genotyping was conducted by TaqMan assay. Whole blood from 31 healthy volunteers was stimulated with LPS (100 ng/mL) to determine the level of IL-10 expression for each allele by enzyme-linked immunosorbent assay (ELISA). Results: After adjustment for percent total body surface area (TBSA) burned, inhalation injury, age, gender, and race/ethnicity, carriage of 592A and/or 819T was significantly associated (P = 0.014) with a decreased risk for death (adjusted odds ratio: 0.404; 95% CI: 0.197-0.829). As the candidate SNPs were in complete linkage disequilibrium, it was not possible to distinguish which allele was associated with decreased mortality risk. Age, inhalation injury, and full-thickness burn size were significantly associated with increased risk for death. In the LPS stimulated blood of healthy controls, carriage of the -592A and/or -819T allele demonstrated a trend for decreased levels of IL-10 (P = 0.079). Conclusion: Carriage of the 592A and/or 819T allele in the IL-10 promoter appears to reduce the risk for death after burn injury.

AB - Objective: Evaluation of single nucleotide polymorphisms (SNPs) in the interleukin10 promoter (592 and 819) on risk for death after burn injury. Methods: Association between the IL-10 SNPs and outcome after burn injury was evaluated in a cohort of 265 patients from Parkland Hospital, Dallas, TX with ≥15% TBSA burns without non-burn trauma (ISS ≤ 16), traumatic or anoxic brain injury or spinal cord injury, who survived >48 h under an IRB-approved protocol. Clinical data were collected prospectively and genotyping was conducted by TaqMan assay. Whole blood from 31 healthy volunteers was stimulated with LPS (100 ng/mL) to determine the level of IL-10 expression for each allele by enzyme-linked immunosorbent assay (ELISA). Results: After adjustment for percent total body surface area (TBSA) burned, inhalation injury, age, gender, and race/ethnicity, carriage of 592A and/or 819T was significantly associated (P = 0.014) with a decreased risk for death (adjusted odds ratio: 0.404; 95% CI: 0.197-0.829). As the candidate SNPs were in complete linkage disequilibrium, it was not possible to distinguish which allele was associated with decreased mortality risk. Age, inhalation injury, and full-thickness burn size were significantly associated with increased risk for death. In the LPS stimulated blood of healthy controls, carriage of the -592A and/or -819T allele demonstrated a trend for decreased levels of IL-10 (P = 0.079). Conclusion: Carriage of the 592A and/or 819T allele in the IL-10 promoter appears to reduce the risk for death after burn injury.

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