IL-10 polymorphism associated with decreased risk for mortality after burn injury

Ryan M. Huebinger, Fernando Rivera-Chavez, Ling Yu Chang, Ming Mei Liu, Joseph P. Minei, Gary F. Purdue, John L. Hunt, Brett D. Arnoldo, Robert C. Barber

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Objective: Evaluation of single nucleotide polymorphisms (SNPs) in the interleukin10 promoter (592 and 819) on risk for death after burn injury. Methods: Association between the IL-10 SNPs and outcome after burn injury was evaluated in a cohort of 265 patients from Parkland Hospital, Dallas, TX with ≥15% TBSA burns without non-burn trauma (ISS ≤ 16), traumatic or anoxic brain injury or spinal cord injury, who survived >48 h under an IRB-approved protocol. Clinical data were collected prospectively and genotyping was conducted by TaqMan assay. Whole blood from 31 healthy volunteers was stimulated with LPS (100 ng/mL) to determine the level of IL-10 expression for each allele by enzyme-linked immunosorbent assay (ELISA). Results: After adjustment for percent total body surface area (TBSA) burned, inhalation injury, age, gender, and race/ethnicity, carriage of 592A and/or 819T was significantly associated (P = 0.014) with a decreased risk for death (adjusted odds ratio: 0.404; 95% CI: 0.197-0.829). As the candidate SNPs were in complete linkage disequilibrium, it was not possible to distinguish which allele was associated with decreased mortality risk. Age, inhalation injury, and full-thickness burn size were significantly associated with increased risk for death. In the LPS stimulated blood of healthy controls, carriage of the -592A and/or -819T allele demonstrated a trend for decreased levels of IL-10 (P = 0.079). Conclusion: Carriage of the 592A and/or 819T allele in the IL-10 promoter appears to reduce the risk for death after burn injury.

Original languageEnglish (US)
Pages (from-to)e141-e145
JournalJournal of Surgical Research
Volume164
Issue number1
DOIs
StatePublished - Nov 2010

Keywords

  • burn injury
  • genetic association
  • interleukin-10
  • mortality

ASJC Scopus subject areas

  • Surgery

Fingerprint Dive into the research topics of 'IL-10 polymorphism associated with decreased risk for mortality after burn injury'. Together they form a unique fingerprint.

Cite this