IL-4 induces the specific rearrangement of γ1 genes on the expressed and unexpressed chromosomes of lipopolysaccharide-activated normal murine B cells

M. R. Kepron, Y. W. Chen, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Small, resting, surface IgM+/surface IgD+ murine B cells undergo an Ig class switch to IgG1 or IgE after stimulation with LPS and T cell supernatants containing IL-4. To firmly establish the role of IL-4 in the directed switch recombination observed in IgG1-secreting cells, we have 1) used highly purified native IL-4 instead of T cell supernatants, 2) used resting B cells from F1 mice in which the active IgH allele was determined before culture, 3) taken advantage of the allelic differences in the restriction fragment lengths of μ, γ1, γ2b, and γ3 loci to determine the status of the C(H) genes on both the expressed and unexpressed chromosomes, and 4) used different restriction enzymes to distinguish between deletion and rearrangement of a given C(H) gene. Our results indicate that LPS alone induces rearrangement of the γ3 genes on both chromosomes wheras stimulation with LPS plus IL-4 results in deletion of γ3 genes and rearrangement of γ1 genes on both chromosomes. The studies definitively establish the role of IL-4 in directed switch recombination to the γ1 locus in LPS-stimulated murine B cells.

Original languageEnglish (US)
Pages (from-to)334-339
Number of pages6
JournalJournal of Immunology
Volume143
Issue number1
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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