IL-7 upregulates T cell receptor/CD3 expression by cultured dendritic epidermal T cells

Mariko Ono, Kiyoshi Ariizumi, Paul R. Bergstresser, Akira Takashima

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Dendritic epidermal T Cells (DETC) in adult mice express uniformly the phenotype of Thy-1+, T cell receptor (TCR)-Vγ3/Vδ1+, CD3+, CD4- and CD8-. In newborn mice, however, the epidermis contains smaller numbers of Thy-1+ cells and they rarely express TCR or CD3, suggesting that a phenotypic maturation and/or a rapid expansion of TCR+/CD3+ cells must occur within the epidermis soon after birth. We have observed previously that keratinocytes produce biologically relevant amounts of IL-7 and that this cytokine promotes the survival and growth of DETC in vitro. Here we report that IL-7 also promotes CD3/TCR expression by DETC. When the long-term cultured DETC line, 7-17, was incubated with IL-7, surface expression was upregulated in time- and dose-dependent fashions, as assessed with antibodies against γδ TCR, Vγ3 TCR or CD3ε. Among 10 other cytokines tested, only IL-2 was effective. IL-7-dependent upregulation also occurred at the levels of mRNA expression (Northern blot) and protein synthesis (immunoprecipitation). We propose that keratinocyte-derived IL-7 promotes not only the growth, but also the phenotypic maturation of DETC, thereby supporting the intraepidermal development of a DETC network during the neonatal period.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalJournal of Dermatological Science
Volume11
Issue number2
DOIs
StatePublished - Feb 1996

Fingerprint

Interleukin-7
T-cells
T-Cell Antigen Receptor
Up-Regulation
T-Lymphocytes
Keratinocytes
Epidermis
Cytokines
Growth
Immunoprecipitation
Northern Blotting
Interleukin-2
Parturition
Phenotype
Cell Line
Messenger RNA
Antibodies
Proteins

Keywords

  • CD3
  • Dendritic epidermal T cell
  • IL-7
  • T cell receptor

ASJC Scopus subject areas

  • Dermatology

Cite this

IL-7 upregulates T cell receptor/CD3 expression by cultured dendritic epidermal T cells. / Ono, Mariko; Ariizumi, Kiyoshi; Bergstresser, Paul R.; Takashima, Akira.

In: Journal of Dermatological Science, Vol. 11, No. 2, 02.1996, p. 89-96.

Research output: Contribution to journalArticle

Ono, Mariko ; Ariizumi, Kiyoshi ; Bergstresser, Paul R. ; Takashima, Akira. / IL-7 upregulates T cell receptor/CD3 expression by cultured dendritic epidermal T cells. In: Journal of Dermatological Science. 1996 ; Vol. 11, No. 2. pp. 89-96.
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