Abstract
Dendritic epidermal T Cells (DETC) in adult mice express uniformly the phenotype of Thy-1+, T cell receptor (TCR)-Vγ3/Vδ1+, CD3+, CD4- and CD8-. In newborn mice, however, the epidermis contains smaller numbers of Thy-1+ cells and they rarely express TCR or CD3, suggesting that a phenotypic maturation and/or a rapid expansion of TCR+/CD3+ cells must occur within the epidermis soon after birth. We have observed previously that keratinocytes produce biologically relevant amounts of IL-7 and that this cytokine promotes the survival and growth of DETC in vitro. Here we report that IL-7 also promotes CD3/TCR expression by DETC. When the long-term cultured DETC line, 7-17, was incubated with IL-7, surface expression was upregulated in time- and dose-dependent fashions, as assessed with antibodies against γδ TCR, Vγ3 TCR or CD3ε. Among 10 other cytokines tested, only IL-2 was effective. IL-7-dependent upregulation also occurred at the levels of mRNA expression (Northern blot) and protein synthesis (immunoprecipitation). We propose that keratinocyte-derived IL-7 promotes not only the growth, but also the phenotypic maturation of DETC, thereby supporting the intraepidermal development of a DETC network during the neonatal period.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 89-96 |
| Number of pages | 8 |
| Journal | Journal of Dermatological Science |
| Volume | 11 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1996 |
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Keywords
- CD3
- Dendritic epidermal T cell
- IL-7
- T cell receptor
ASJC Scopus subject areas
- Dermatology
Cite this
IL-7 upregulates T cell receptor/CD3 expression by cultured dendritic epidermal T cells. / Ono, Mariko; Ariizumi, Kiyoshi; Bergstresser, Paul R.; Takashima, Akira.
In: Journal of Dermatological Science, Vol. 11, No. 2, 02.1996, p. 89-96.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - IL-7 upregulates T cell receptor/CD3 expression by cultured dendritic epidermal T cells
AU - Ono, Mariko
AU - Ariizumi, Kiyoshi
AU - Bergstresser, Paul R.
AU - Takashima, Akira
PY - 1996/2
Y1 - 1996/2
N2 - Dendritic epidermal T Cells (DETC) in adult mice express uniformly the phenotype of Thy-1+, T cell receptor (TCR)-Vγ3/Vδ1+, CD3+, CD4- and CD8-. In newborn mice, however, the epidermis contains smaller numbers of Thy-1+ cells and they rarely express TCR or CD3, suggesting that a phenotypic maturation and/or a rapid expansion of TCR+/CD3+ cells must occur within the epidermis soon after birth. We have observed previously that keratinocytes produce biologically relevant amounts of IL-7 and that this cytokine promotes the survival and growth of DETC in vitro. Here we report that IL-7 also promotes CD3/TCR expression by DETC. When the long-term cultured DETC line, 7-17, was incubated with IL-7, surface expression was upregulated in time- and dose-dependent fashions, as assessed with antibodies against γδ TCR, Vγ3 TCR or CD3ε. Among 10 other cytokines tested, only IL-2 was effective. IL-7-dependent upregulation also occurred at the levels of mRNA expression (Northern blot) and protein synthesis (immunoprecipitation). We propose that keratinocyte-derived IL-7 promotes not only the growth, but also the phenotypic maturation of DETC, thereby supporting the intraepidermal development of a DETC network during the neonatal period.
AB - Dendritic epidermal T Cells (DETC) in adult mice express uniformly the phenotype of Thy-1+, T cell receptor (TCR)-Vγ3/Vδ1+, CD3+, CD4- and CD8-. In newborn mice, however, the epidermis contains smaller numbers of Thy-1+ cells and they rarely express TCR or CD3, suggesting that a phenotypic maturation and/or a rapid expansion of TCR+/CD3+ cells must occur within the epidermis soon after birth. We have observed previously that keratinocytes produce biologically relevant amounts of IL-7 and that this cytokine promotes the survival and growth of DETC in vitro. Here we report that IL-7 also promotes CD3/TCR expression by DETC. When the long-term cultured DETC line, 7-17, was incubated with IL-7, surface expression was upregulated in time- and dose-dependent fashions, as assessed with antibodies against γδ TCR, Vγ3 TCR or CD3ε. Among 10 other cytokines tested, only IL-2 was effective. IL-7-dependent upregulation also occurred at the levels of mRNA expression (Northern blot) and protein synthesis (immunoprecipitation). We propose that keratinocyte-derived IL-7 promotes not only the growth, but also the phenotypic maturation of DETC, thereby supporting the intraepidermal development of a DETC network during the neonatal period.
KW - CD3
KW - Dendritic epidermal T cell
KW - IL-7
KW - T cell receptor
UR - http://www.scopus.com/inward/record.url?scp=0030058556&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030058556&partnerID=8YFLogxK
U2 - 10.1016/0923-1811(95)00424-6
DO - 10.1016/0923-1811(95)00424-6
M3 - Article
VL - 11
SP - 89
EP - 96
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 2
ER -