TY - JOUR
T1 - Image analysis reveals differences in tumor multinucleations in Black and White patients with human papillomavirus-associated oropharyngeal squamous cell carcinoma
AU - Koyuncu, Can F.
AU - Nag, Reetoja
AU - Lu, Cheng
AU - Corredor, Germán
AU - Viswanathan, Vidya S.
AU - Sandulache, Vlad C.
AU - Fu, Pingfu
AU - Yang, Kailin
AU - Pan, Quintin
AU - Zhang, Zelin
AU - Xu, Jun
AU - Chute, Deborah J.
AU - Thorstad, Wade L.
AU - Faraji, Farhoud
AU - Bishop, Justin A.
AU - Mehrad, Mitra
AU - Castro, Patricia D.
AU - Sikora, Andrew G.
AU - Thompson, Lester D.R.
AU - Chernock, Rebecca D.
AU - Lang Kuhs, Krystle A.
AU - Wasman, Jay K.
AU - Luo, Jingqin R.
AU - Adelstein, David J.
AU - Koyfman, Shlomo A.
AU - Lewis, James S.
AU - Madabhushi, Anant
N1 - Publisher Copyright:
© 2022 The Authors. Cancer published by Wiley Periodicals LLC on behalf of American Cancer Society.
PY - 2022/11/1
Y1 - 2022/11/1
N2 - Background: Understanding biological differences between different racial groups of human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) patients, who have differences in terms of incidence, survival, and tumor morphology, can facilitate accurate prognostic biomarkers, which can help develop personalized treatment strategies. Methods: This study evaluated whether there were morphologic differences between HPV-associated tumors from Black and White patients in terms of multinucleation index (MuNI), an image analysis-derived metric that measures density of multinucleated tumor cells within epithelial regions on hematoxylin–eosin images and previously has been prognostic in HPV-associated OPSCC patients. In this study, the authors specifically evaluated whether the same MuNI cutoff that was prognostic of overall survival (OS) and disease-free survival in their previous study, TTR, is valid for Black and White patients, separately. We also evaluated population-specific cutoffs, TB for Blacks and TW for Whites, for risk stratification. Results: MuNI was statistically significantly different between Black (mean, 3.88e–4; median, 3.67e–04) and White patients (mean, 3.36e–04; median, 2.99e–04), with p =.0078. Using TTR, MuNI was prognostic of OS in the entire population with hazard ratio (HR) of 1.71 (p =.002; 95% confidence interval [CI], 1.21–2.43) and in White patients with HR of 1.72 (p =.005; 95% CI, 1.18–2.51). Population-specific cutoff, TW, yielded improved HR of 1.77 (p =.003; 95% CI, 1.21–2.58) for White patients, whereas TB did not improve risk-stratification in Black patients with HR of 0.6 (p =.3; HR, 0.6; 95% CI, 0.2–1.80). Conclusions: Histological difference between White and Black patient tumors in terms of multinucleated tumor cells suggests the need for considering population-specific prognostic biomarkers for personalized risk stratification strategies for HPV-associated OPSCC patients.
AB - Background: Understanding biological differences between different racial groups of human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) patients, who have differences in terms of incidence, survival, and tumor morphology, can facilitate accurate prognostic biomarkers, which can help develop personalized treatment strategies. Methods: This study evaluated whether there were morphologic differences between HPV-associated tumors from Black and White patients in terms of multinucleation index (MuNI), an image analysis-derived metric that measures density of multinucleated tumor cells within epithelial regions on hematoxylin–eosin images and previously has been prognostic in HPV-associated OPSCC patients. In this study, the authors specifically evaluated whether the same MuNI cutoff that was prognostic of overall survival (OS) and disease-free survival in their previous study, TTR, is valid for Black and White patients, separately. We also evaluated population-specific cutoffs, TB for Blacks and TW for Whites, for risk stratification. Results: MuNI was statistically significantly different between Black (mean, 3.88e–4; median, 3.67e–04) and White patients (mean, 3.36e–04; median, 2.99e–04), with p =.0078. Using TTR, MuNI was prognostic of OS in the entire population with hazard ratio (HR) of 1.71 (p =.002; 95% confidence interval [CI], 1.21–2.43) and in White patients with HR of 1.72 (p =.005; 95% CI, 1.18–2.51). Population-specific cutoff, TW, yielded improved HR of 1.77 (p =.003; 95% CI, 1.21–2.58) for White patients, whereas TB did not improve risk-stratification in Black patients with HR of 0.6 (p =.3; HR, 0.6; 95% CI, 0.2–1.80). Conclusions: Histological difference between White and Black patient tumors in terms of multinucleated tumor cells suggests the need for considering population-specific prognostic biomarkers for personalized risk stratification strategies for HPV-associated OPSCC patients.
KW - Black
KW - HPV
KW - MuNI
KW - OPSCC
KW - White
KW - image analysis
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U2 - 10.1002/cncr.34446
DO - 10.1002/cncr.34446
M3 - Article
C2 - 36066461
AN - SCOPUS:85138273581
SN - 0008-543X
VL - 128
SP - 3831
EP - 3842
JO - Cancer
JF - Cancer
IS - 21
ER -