Imaging β-Cell Function Using a Zinc-Responsive MRI Contrast Agent May Identify First Responder Islets

Bibek Thapa, Eul Hyun Suh, Daniel Parrott, Pooyan Khalighinejad, Gaurav Sharma, Sara Chirayil, A. Dean Sherry

Research output: Contribution to journalArticlepeer-review

Abstract

An imaging method for detecting β-cell function in real-time in the rodent pancreas could provide new insights into the biological mechanisms involving loss of β-cell function during development of type 2 diabetes and for testing of new drugs designed to modulate insulin secretion. In this study, we used a zinc-responsive MRI contrast agent and an optimized 2D MRI method to show that glucose stimulated insulin and zinc secretion can be detected as functionally active “hot spots” in the tail of the rat pancreas. A comparison of functional images with histological markers show that insulin and zinc secretion does not occur uniformly among all pancreatic islets but rather that some islets respond rapidly to an increase in glucose while others remain silent. Zinc and insulin secretion was shown to be altered in streptozotocin and exenatide treated rats thereby verifying that this simple MRI technique is responsive to changes in β-cell function.

Original languageEnglish (US)
Article number809867
JournalFrontiers in Endocrinology
Volume12
DOIs
StatePublished - Jan 31 2022

Keywords

  • glucose stimulated zinc secretion (GSZS)
  • glucose-stimulated insulin secretion (GSIS)
  • magnetic resonance imaging
  • metabolic imaging
  • pancreatic β-cell function
  • zinc-responsive contrast agent

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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