Imaging and modeling the dynamics of clathrin-mediated endocytosis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Clathrin-mediated endocytosis (CME) plays a central role in cellular homeostasis and is mediated by clathrin-coated pits (CCPs). Live-cell imaging has revealed a remarkable heterogeneity in CCPassembly kinetics, which can be used as an intrinsic source of mechanistic information on CCP regulation but also poses several major problems for unbiased analysis of CME dynamics. The backbone of unveiling the molecular control of CME is an imagingbased inventory of the full diversity of individual CCP behaviors, which requires detection and tracking of structural fiduciaries and regulatory proteins with an accuracy of >99.9%, despite very low signals. This level of confidence can only be achieved by combining appropriate imaging modalities with self-diagnostic computational algorithms for image analysis and data mining.

Original languageEnglish (US)
Article numbera017038
JournalCold Spring Harbor perspectives in biology
Volume6
Issue number12
DOIs
StatePublished - Dec 1 2014

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Clathrin
Endocytosis
Imaging techniques
Data Mining
Image analysis
Data mining
Homeostasis
Equipment and Supplies
Kinetics
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Imaging and modeling the dynamics of clathrin-mediated endocytosis. / Mettlen, Marcel; Danuser, Gaudenz.

In: Cold Spring Harbor perspectives in biology, Vol. 6, No. 12, a017038, 01.12.2014.

Research output: Contribution to journalArticle

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