Imaging dynamic insulin release using a fluorescent zinc indicator for monitoring induced exocytotic release (ZIMIR)

Daliang Li, Shiuhwei Chen, Elisa A. Bellomo, Andrei I. Tarasov, Callan Kaut, Guy A. Rutter, Wen Hong Li

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Current methods of monitoring insulin secretion lack the required spatial and temporal resolution to adequately map the dynamics of exocytosis of native insulin granules in intact cell populations in three dimensions. Exploiting the fact that insulin granules contain a high level of Zn 2+, and that Zn 2+ is coreleased with insulin during secretion, we have developed a fluorescent, cell surface-targeted zinc indicator for monitoring induced exocytotic release (ZIMIR). ZIMIR displayed a robust fluorescence enhancement on Zn 2+ chelation and bound Zn 2+ with high selectivity against Ca 2+ and Mg 2+. When added to cultured β cells or intact pancreatic islets at low micromolar concentrations, ZIMIR labeled cells rapidly, noninvasively, and stably, and it reliably reported changes in Zn 2+ concentration near the sites of granule fusion with high sensitivity that correlated well with membrane capacitance measurement. Fluorescence imaging of ZIMIR-labeled β cells followed the dynamics of exocytotic activity at subcellular resolution, even when using simple epifluorescence microscopy, and located the chief sites of insulin release to intercellular junctions. Moreover, ZIMIR imaging of intact rat islets revealed that Zn 2+/insulin release occurred largely in small groups of adjacent β cells, with each forming a "secretory unit." Concurrent imaging of ZIMIR and Fura-2 showed that the amplitude of cytosolic Ca 2+ elevation did not necessarily correlate with insulin secretion activity, suggesting that events downstream of Ca 2+ signaling underlie the cell-cell heterogeneity in insulin release. In addition to studying stimulation-secretion coupling in cells with Zn 2+-containing granules, ZIMIR may find applications in β-cell engineering and screening for molecules regulating insulin secretion on high-throughput platforms.

Original languageEnglish (US)
Pages (from-to)21063-21068
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number52
DOIs
StatePublished - Dec 27 2011

Fingerprint

Zinc
Insulin
Cell Engineering
Intercellular Junctions
Fura-2
Optical Imaging
Exocytosis
Islets of Langerhans
Microscopy
Cultured Cells
Fluorescence
Membranes
Population

Keywords

  • Hormone secretion assay
  • Probe development
  • Zinc imaging

ASJC Scopus subject areas

  • General

Cite this

Imaging dynamic insulin release using a fluorescent zinc indicator for monitoring induced exocytotic release (ZIMIR). / Li, Daliang; Chen, Shiuhwei; Bellomo, Elisa A.; Tarasov, Andrei I.; Kaut, Callan; Rutter, Guy A.; Li, Wen Hong.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 52, 27.12.2011, p. 21063-21068.

Research output: Contribution to journalArticle

Li, Daliang ; Chen, Shiuhwei ; Bellomo, Elisa A. ; Tarasov, Andrei I. ; Kaut, Callan ; Rutter, Guy A. ; Li, Wen Hong. / Imaging dynamic insulin release using a fluorescent zinc indicator for monitoring induced exocytotic release (ZIMIR). In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 52. pp. 21063-21068.
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