Imaging Lung Clearance of Radiolabeled Tumor Cells to Study Mice with Normal, Activated or Depleted Natural Killer (NK) Cells

P. V. Kulkarni, M. Bennett, A. Constantinescu, V. Arora, M. Viguet, P. Antich, R. W. Parkey, D. Mathews, R. P. Mason, O. K. Oz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Lung clearance of 51CR and 125I iododeoxyuridine (IUDR) labeled cancer cells assess NK cell activity. It is desirable to develop noninvasive imaging technique to assess NK activity in mice. We labeled target YAC-1 tumor cells with 125I, 111In, 99mTc, or 67Ga and injected I.V. into three groups of BALB/c mice. Animals were treated with medium (group I), 300mg/kg cyclophosmamide (CY) to kill NK cell (group II), or anti-LY49C/1) (ab')2 mAb to augment NK function (group III). Lungs were removed 15 min or 2 h later for tissue counting. Control and treated mice were imaged every 5 min with a scintillating camera for 1 h after 15 min of infusion of the 111In labeled cells. Lung clearance increased after 15 min (lodging: 60-80%) and (2 h retention: 3-7%). Similar results were obtained with all the isotopes studied. Images distinguished the control and treated mice for lung activity. Cells labeled with 111In, 99mTc or 67Ga are cleared similar to those labeled with 51Cr or 125I. NK cell destruction of tumor cells may be assessed by noninvasive imaging method either by SPECT (99mTc, 111In, 67Ga) or by PET (68Ga).

Original languageEnglish (US)
Title of host publicationApplication of Accelerators in Research and Industry
Subtitle of host publication17th International Conference on the Application of Accelerators in Research and Industry
PublisherAmerican Institute of Physics Inc.
Pages1125-1128
Number of pages4
Volume680
ISBN (Electronic)0735401497
DOIs
Publication statusPublished - Aug 26 2003
Event17th International Conference on the Application of Accelerators in Research and Industry - Denton, United States
Duration: Nov 12 2002Nov 16 2002

Other

Other17th International Conference on the Application of Accelerators in Research and Industry
CountryUnited States
CityDenton
Period11/12/0211/16/02

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ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Kulkarni, P. V., Bennett, M., Constantinescu, A., Arora, V., Viguet, M., Antich, P., ... Oz, O. K. (2003). Imaging Lung Clearance of Radiolabeled Tumor Cells to Study Mice with Normal, Activated or Depleted Natural Killer (NK) Cells. In Application of Accelerators in Research and Industry: 17th International Conference on the Application of Accelerators in Research and Industry (Vol. 680, pp. 1125-1128). American Institute of Physics Inc.. https://doi.org/10.1063/1.1619906