Imaging of fluorescently tagged ATM kinase at the sites of DNA double strand breaks

Anthony J. Davis, Shih Ya Wang, David J. Chen, Benjamin P.C. Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The combination of live cell imaging and laser micro-irradiation is an important technique to investigate the recruitment and kinetics of DNA repair molecules to DNA damage sites. In this chapter, we describe the detailed methods to study the dynamics of fluorescently tagged ATM protein kinase at laser-induced DNA double strand break (DSB) sites. The same protocol can be applied to analyze the recruitment and kinetics of other potential or known DNA repair proteins to DSB sites.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-285
Number of pages9
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1599
ISSN (Print)1064-3745

Keywords

  • ATM kinase
  • DNA double strand breaks
  • Green fluorescent protein
  • Laser micro-irradiation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Davis, A. J., Wang, S. Y., Chen, D. J., & Chen, B. P. C. (2017). Imaging of fluorescently tagged ATM kinase at the sites of DNA double strand breaks. In Methods in Molecular Biology (pp. 277-285). (Methods in Molecular Biology; Vol. 1599). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6955-5_20