Imaging of homeostatic, neoplastic, and injured tissues by HA-based probes

Mandana Veiseh, Daniel Breadner, Jenny Ma, Natalia Akentieva, Rashmin C. Savani, Rene Harrison, David Mikilus, Lisa Collis, Stefan Gustafson, Ting Yim Lee, James Koropatnick, Leonard G. Luyt, Mina J. Bissell, Eva A. Turley

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism, thus providing a flexible imaging strategy for monitoring disease and repair processes. Kinetic analyses confirmed favorable available serum levels of the probe following intravenous (i.v.) or subcutaneous (s.c.) injection. Nuclear (technetium-HA, 99mTc-HA, and iodine-HA, 125I-HA), optical (fluorescent Texas Red-HA, TR-HA), and magnetic resonance (gadolinium-HA, Gd-HA) probes imaged liver ( 99mTc-HA), breast cancer cells/xenografts (TR-HA, Gd-HA), and vascular injury ( 125I-HA, TR-HA). Targeting of HA probes to these sites appeared to result from selective HA receptor-dependent localization. Our results suggest that HA-based probes, which do not require polysaccharide backbone modification to achieve favorable half-life and distribution, can detect elevated HA metabolism in homeostatic, injured, and diseased tissues.

Original languageEnglish (US)
Pages (from-to)12-22
Number of pages11
JournalBiomacromolecules
Volume13
Issue number1
DOIs
StatePublished - Jan 9 2012

Fingerprint

Hyaluronic Acid
Tissue
Imaging techniques
Metabolism
Gadolinium
Technetium
Magnetic resonance
Polysaccharides
Iodine
Liver
Repair
Cells
Kinetics
Monitoring
CD44 Antigens
Heterografts

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Veiseh, M., Breadner, D., Ma, J., Akentieva, N., Savani, R. C., Harrison, R., ... Turley, E. A. (2012). Imaging of homeostatic, neoplastic, and injured tissues by HA-based probes. Biomacromolecules, 13(1), 12-22. https://doi.org/10.1021/bm201143c

Imaging of homeostatic, neoplastic, and injured tissues by HA-based probes. / Veiseh, Mandana; Breadner, Daniel; Ma, Jenny; Akentieva, Natalia; Savani, Rashmin C.; Harrison, Rene; Mikilus, David; Collis, Lisa; Gustafson, Stefan; Lee, Ting Yim; Koropatnick, James; Luyt, Leonard G.; Bissell, Mina J.; Turley, Eva A.

In: Biomacromolecules, Vol. 13, No. 1, 09.01.2012, p. 12-22.

Research output: Contribution to journalArticle

Veiseh, M, Breadner, D, Ma, J, Akentieva, N, Savani, RC, Harrison, R, Mikilus, D, Collis, L, Gustafson, S, Lee, TY, Koropatnick, J, Luyt, LG, Bissell, MJ & Turley, EA 2012, 'Imaging of homeostatic, neoplastic, and injured tissues by HA-based probes', Biomacromolecules, vol. 13, no. 1, pp. 12-22. https://doi.org/10.1021/bm201143c
Veiseh, Mandana ; Breadner, Daniel ; Ma, Jenny ; Akentieva, Natalia ; Savani, Rashmin C. ; Harrison, Rene ; Mikilus, David ; Collis, Lisa ; Gustafson, Stefan ; Lee, Ting Yim ; Koropatnick, James ; Luyt, Leonard G. ; Bissell, Mina J. ; Turley, Eva A. / Imaging of homeostatic, neoplastic, and injured tissues by HA-based probes. In: Biomacromolecules. 2012 ; Vol. 13, No. 1. pp. 12-22.
@article{bd75e04b30f54552860b07e0db908a62,
title = "Imaging of homeostatic, neoplastic, and injured tissues by HA-based probes",
abstract = "An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism, thus providing a flexible imaging strategy for monitoring disease and repair processes. Kinetic analyses confirmed favorable available serum levels of the probe following intravenous (i.v.) or subcutaneous (s.c.) injection. Nuclear (technetium-HA, 99mTc-HA, and iodine-HA, 125I-HA), optical (fluorescent Texas Red-HA, TR-HA), and magnetic resonance (gadolinium-HA, Gd-HA) probes imaged liver ( 99mTc-HA), breast cancer cells/xenografts (TR-HA, Gd-HA), and vascular injury ( 125I-HA, TR-HA). Targeting of HA probes to these sites appeared to result from selective HA receptor-dependent localization. Our results suggest that HA-based probes, which do not require polysaccharide backbone modification to achieve favorable half-life and distribution, can detect elevated HA metabolism in homeostatic, injured, and diseased tissues.",
author = "Mandana Veiseh and Daniel Breadner and Jenny Ma and Natalia Akentieva and Savani, {Rashmin C.} and Rene Harrison and David Mikilus and Lisa Collis and Stefan Gustafson and Lee, {Ting Yim} and James Koropatnick and Luyt, {Leonard G.} and Bissell, {Mina J.} and Turley, {Eva A.}",
year = "2012",
month = "1",
day = "9",
doi = "10.1021/bm201143c",
language = "English (US)",
volume = "13",
pages = "12--22",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "1",

}

TY - JOUR

T1 - Imaging of homeostatic, neoplastic, and injured tissues by HA-based probes

AU - Veiseh, Mandana

AU - Breadner, Daniel

AU - Ma, Jenny

AU - Akentieva, Natalia

AU - Savani, Rashmin C.

AU - Harrison, Rene

AU - Mikilus, David

AU - Collis, Lisa

AU - Gustafson, Stefan

AU - Lee, Ting Yim

AU - Koropatnick, James

AU - Luyt, Leonard G.

AU - Bissell, Mina J.

AU - Turley, Eva A.

PY - 2012/1/9

Y1 - 2012/1/9

N2 - An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism, thus providing a flexible imaging strategy for monitoring disease and repair processes. Kinetic analyses confirmed favorable available serum levels of the probe following intravenous (i.v.) or subcutaneous (s.c.) injection. Nuclear (technetium-HA, 99mTc-HA, and iodine-HA, 125I-HA), optical (fluorescent Texas Red-HA, TR-HA), and magnetic resonance (gadolinium-HA, Gd-HA) probes imaged liver ( 99mTc-HA), breast cancer cells/xenografts (TR-HA, Gd-HA), and vascular injury ( 125I-HA, TR-HA). Targeting of HA probes to these sites appeared to result from selective HA receptor-dependent localization. Our results suggest that HA-based probes, which do not require polysaccharide backbone modification to achieve favorable half-life and distribution, can detect elevated HA metabolism in homeostatic, injured, and diseased tissues.

AB - An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism, thus providing a flexible imaging strategy for monitoring disease and repair processes. Kinetic analyses confirmed favorable available serum levels of the probe following intravenous (i.v.) or subcutaneous (s.c.) injection. Nuclear (technetium-HA, 99mTc-HA, and iodine-HA, 125I-HA), optical (fluorescent Texas Red-HA, TR-HA), and magnetic resonance (gadolinium-HA, Gd-HA) probes imaged liver ( 99mTc-HA), breast cancer cells/xenografts (TR-HA, Gd-HA), and vascular injury ( 125I-HA, TR-HA). Targeting of HA probes to these sites appeared to result from selective HA receptor-dependent localization. Our results suggest that HA-based probes, which do not require polysaccharide backbone modification to achieve favorable half-life and distribution, can detect elevated HA metabolism in homeostatic, injured, and diseased tissues.

UR - http://www.scopus.com/inward/record.url?scp=84862915161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862915161&partnerID=8YFLogxK

U2 - 10.1021/bm201143c

DO - 10.1021/bm201143c

M3 - Article

VL - 13

SP - 12

EP - 22

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 1

ER -