Imaging two targets in live cells based on rational design of lanthanide organic structure appended carbon dots

Zhan Zhou; Qianming Wang; Jinyong Wang; Chengcheng Zhang

doi:10.1016/j.carbon.2015.05.107

Imaging two targets in live cells based on rational design of lanthanide organic structure appended carbon dots

Zhan Zhou, Qianming Wang, Jinyong Wang, Chengcheng Zhang

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

New chemical sensors based on lanthanide emission that enable sequential detection of two targets in live cells have been developed. The indicator molecules (unsaturated europium complexes) were covalently immobilized on carbon quantum dots (CQDs), and a water soluble fluorescent nano-probe (CQDs-DTPA-Eu) with biocompatibility was fabricated. A novel stepwise assembly method was used to generate unique responses to two different targets sequentially. Rapid and highly selective detection of tetracycline (Tc) in water and milk (detection limit 32 nM) were demonstrated. The in situ generated CQDs-DTPA-EuTc was non-emissive, but, upon addition of hydrogen peroxide (detection limit 21 nM) intense red luminescence was observed. Highly reproducible results were obtained in Plat-E cells, demonstrating the identification of tetracycline and H₂O₂ in cellular environment with high accuracy.

Original language	English (US)
Pages (from-to)	671-680
Number of pages	10
Journal	Carbon
Volume	93
DOIs	https://doi.org/10.1016/j.carbon.2015.05.107
State	Published - Aug 8 2015

ASJC Scopus subject areas

General Chemistry
General Materials Science

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10.1016/j.carbon.2015.05.107

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title = "Imaging two targets in live cells based on rational design of lanthanide organic structure appended carbon dots",

abstract = "New chemical sensors based on lanthanide emission that enable sequential detection of two targets in live cells have been developed. The indicator molecules (unsaturated europium complexes) were covalently immobilized on carbon quantum dots (CQDs), and a water soluble fluorescent nano-probe (CQDs-DTPA-Eu) with biocompatibility was fabricated. A novel stepwise assembly method was used to generate unique responses to two different targets sequentially. Rapid and highly selective detection of tetracycline (Tc) in water and milk (detection limit 32 nM) were demonstrated. The in situ generated CQDs-DTPA-EuTc was non-emissive, but, upon addition of hydrogen peroxide (detection limit 21 nM) intense red luminescence was observed. Highly reproducible results were obtained in Plat-E cells, demonstrating the identification of tetracycline and H2O2 in cellular environment with high accuracy.",

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journal = "Carbon",

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T1 - Imaging two targets in live cells based on rational design of lanthanide organic structure appended carbon dots

AU - Zhou, Zhan

AU - Wang, Qianming

AU - Wang, Jinyong

AU - Zhang, Chengcheng

PY - 2015/8/8

Y1 - 2015/8/8

N2 - New chemical sensors based on lanthanide emission that enable sequential detection of two targets in live cells have been developed. The indicator molecules (unsaturated europium complexes) were covalently immobilized on carbon quantum dots (CQDs), and a water soluble fluorescent nano-probe (CQDs-DTPA-Eu) with biocompatibility was fabricated. A novel stepwise assembly method was used to generate unique responses to two different targets sequentially. Rapid and highly selective detection of tetracycline (Tc) in water and milk (detection limit 32 nM) were demonstrated. The in situ generated CQDs-DTPA-EuTc was non-emissive, but, upon addition of hydrogen peroxide (detection limit 21 nM) intense red luminescence was observed. Highly reproducible results were obtained in Plat-E cells, demonstrating the identification of tetracycline and H2O2 in cellular environment with high accuracy.

AB - New chemical sensors based on lanthanide emission that enable sequential detection of two targets in live cells have been developed. The indicator molecules (unsaturated europium complexes) were covalently immobilized on carbon quantum dots (CQDs), and a water soluble fluorescent nano-probe (CQDs-DTPA-Eu) with biocompatibility was fabricated. A novel stepwise assembly method was used to generate unique responses to two different targets sequentially. Rapid and highly selective detection of tetracycline (Tc) in water and milk (detection limit 32 nM) were demonstrated. The in situ generated CQDs-DTPA-EuTc was non-emissive, but, upon addition of hydrogen peroxide (detection limit 21 nM) intense red luminescence was observed. Highly reproducible results were obtained in Plat-E cells, demonstrating the identification of tetracycline and H2O2 in cellular environment with high accuracy.

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JO - Carbon

JF - Carbon

ER -

Imaging two targets in live cells based on rational design of lanthanide organic structure appended carbon dots

Abstract

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