Immediate pro-inflammatory cytokine response associated with asphyxia and volume infusions in a neonatal piglet model

Lina F. Chalak, Abbot R. Laptook, Jeffrey M. Perlman, Damian Garcia, Octavio Ramilo, Myra H. Wyckoff

Research output: Contribution to journalArticlepeer-review

Abstract

A neonatal piglet model was used to determine IL6, IL8 and CRP responses to asphyxia and subsequent volume infusion. Mechanically ventilated swine (n = 37, age: 8 ± 2 days, weight: 2.2 ± 0.7 kg) were progressively asphyxiated by changing the ventilator gases until heart rate <100 bpm and mean arterial blood pressure <20 mmHg. After 5 minutes of ventilatory resuscitation, piglets were randomized to receive volume infusion with either 5% Albumin (ALB), Normal Saline (NS), or no volume (SHAM). IL6, IL8 and CRP were measured at baseline, at the end of asphyxia (ASPYHXIA), and 2 hrs after resuscitation (RESUSCITATION). IL6 (median and [25-75 percentiles] pg/ml) increased from 163 [55,193] at baseline, to 263 [196,393] at ASPHYXIA, and 441 [309,875] at RESUSCITATION (p < 0.001). Both IL8 and CRP were similar to baseline at all time intervals. Animals randomized to volume (ALB and NS) vs. SHAM had higher IL6 levels following RESUSCITATION (749 [383,1163] vs. 303 [204,465], p < 0.02). In this model of severe neonatal asphyxia, serum IL-6 was elevated following volume infusion. The lack of change in IL8 and CRP suggests selectivity in the immediate inflammatory responses following asphyxia and volume resuscitation.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalJournal of Neonatal-Perinatal Medicine
Volume1
Issue number2
StatePublished - 2008

Keywords

  • Asphyxia
  • CRP
  • Interleukins
  • Newborn
  • Resuscitation
  • Volume expansion

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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