TY - JOUR
T1 - Immediate pro-inflammatory cytokine response associated with asphyxia and volume infusions in a neonatal piglet model
AU - Chalak, Lina F.
AU - Laptook, Abbot R.
AU - Perlman, Jeffrey M.
AU - Garcia, Damian
AU - Ramilo, Octavio
AU - Wyckoff, Myra H.
PY - 2008
Y1 - 2008
N2 - A neonatal piglet model was used to determine IL6, IL8 and CRP responses to asphyxia and subsequent volume infusion. Mechanically ventilated swine (n = 37, age: 8 ± 2 days, weight: 2.2 ± 0.7 kg) were progressively asphyxiated by changing the ventilator gases until heart rate <100 bpm and mean arterial blood pressure <20 mmHg. After 5 minutes of ventilatory resuscitation, piglets were randomized to receive volume infusion with either 5% Albumin (ALB), Normal Saline (NS), or no volume (SHAM). IL6, IL8 and CRP were measured at baseline, at the end of asphyxia (ASPYHXIA), and 2 hrs after resuscitation (RESUSCITATION). IL6 (median and [25-75 percentiles] pg/ml) increased from 163 [55,193] at baseline, to 263 [196,393] at ASPHYXIA, and 441 [309,875] at RESUSCITATION (p < 0.001). Both IL8 and CRP were similar to baseline at all time intervals. Animals randomized to volume (ALB and NS) vs. SHAM had higher IL6 levels following RESUSCITATION (749 [383,1163] vs. 303 [204,465], p < 0.02). In this model of severe neonatal asphyxia, serum IL-6 was elevated following volume infusion. The lack of change in IL8 and CRP suggests selectivity in the immediate inflammatory responses following asphyxia and volume resuscitation.
AB - A neonatal piglet model was used to determine IL6, IL8 and CRP responses to asphyxia and subsequent volume infusion. Mechanically ventilated swine (n = 37, age: 8 ± 2 days, weight: 2.2 ± 0.7 kg) were progressively asphyxiated by changing the ventilator gases until heart rate <100 bpm and mean arterial blood pressure <20 mmHg. After 5 minutes of ventilatory resuscitation, piglets were randomized to receive volume infusion with either 5% Albumin (ALB), Normal Saline (NS), or no volume (SHAM). IL6, IL8 and CRP were measured at baseline, at the end of asphyxia (ASPYHXIA), and 2 hrs after resuscitation (RESUSCITATION). IL6 (median and [25-75 percentiles] pg/ml) increased from 163 [55,193] at baseline, to 263 [196,393] at ASPHYXIA, and 441 [309,875] at RESUSCITATION (p < 0.001). Both IL8 and CRP were similar to baseline at all time intervals. Animals randomized to volume (ALB and NS) vs. SHAM had higher IL6 levels following RESUSCITATION (749 [383,1163] vs. 303 [204,465], p < 0.02). In this model of severe neonatal asphyxia, serum IL-6 was elevated following volume infusion. The lack of change in IL8 and CRP suggests selectivity in the immediate inflammatory responses following asphyxia and volume resuscitation.
KW - Asphyxia
KW - CRP
KW - Interleukins
KW - Newborn
KW - Resuscitation
KW - Volume expansion
UR - http://www.scopus.com/inward/record.url?scp=85013578691&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85013578691&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85013578691
SN - 1934-5798
VL - 1
SP - 69
EP - 75
JO - Journal of Neonatal-Perinatal Medicine
JF - Journal of Neonatal-Perinatal Medicine
IS - 2
ER -