Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes

Dongmei Han; Carlos A. Leyva; Della Matheson; Davide Mineo; Shari Messinger; Bonnie B. Blomberg; Ana Hernandez; Luigi F. Meneghini; Gloria Allende; Jay S. Skyler; Rodolfo Alejandro; Alberto Pugliese; Norma S. Kenyon

doi:10.1016/j.clim.2011.02.016

Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes

Dongmei Han, Carlos A. Leyva, Della Matheson, Davide Mineo, Shari Messinger, Bonnie B. Blomberg, Ana Hernandez, Luigi F. Meneghini, Gloria Allende, Jay S. Skyler, Rodolfo Alejandro, Alberto Pugliese, Norma S. Kenyon

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26 Scopus citations

Abstract

There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-αalpha;, IFN-γgamma;, Foxp3, IL-10, TGF-βbeta;, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG<gamma>) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-γ, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG<gamma> and up-regulation of IFN-γ with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease.

Original language	English (US)
Pages (from-to)	290-301
Number of pages	12
Journal	Clinical Immunology
Volume	139
Issue number	3
DOIs	https://doi.org/10.1016/j.clim.2011.02.016
State	Published - Jun 2011

Keywords

Age
Biomarkers
Cytokines
Cytotoxic lymphocyte genes
Gene expression
Type 1 diabetes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.clim.2011.02.016

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Han, D., Leyva, C. A., Matheson, D., Mineo, D., Messinger, S., Blomberg, B. B., Hernandez, A., Meneghini, L. F., Allende, G., Skyler, J. S., Alejandro, R., Pugliese, A., & Kenyon, N. S. (2011). Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes. Clinical Immunology, 139(3), 290-301. https://doi.org/10.1016/j.clim.2011.02.016

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title = "Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes",

abstract = "There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-αalpha;, IFN-γgamma;, Foxp3, IL-10, TGF-βbeta;, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-γ, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG and up-regulation of IFN-γ with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease.",

keywords = "Age, Biomarkers, Cytokines, Cytotoxic lymphocyte genes, Gene expression, Type 1 diabetes",

author = "Dongmei Han and Leyva, {Carlos A.} and Della Matheson and Davide Mineo and Shari Messinger and Blomberg, {Bonnie B.} and Ana Hernandez and Meneghini, {Luigi F.} and Gloria Allende and Skyler, {Jay S.} and Rodolfo Alejandro and Alberto Pugliese and Kenyon, {Norma S.}",

note = "Funding Information: This work was supported by the Diabetes Research Institute Foundation, Hollywood, FL, USA and the following grants: National Institutes of Health ( U01 DK061041 , U01 DK061037 , UO1 DK070460 ).",

year = "2011",

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doi = "10.1016/j.clim.2011.02.016",

language = "English (US)",

volume = "139",

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AU - Han, Dongmei

AU - Leyva, Carlos A.

AU - Matheson, Della

AU - Mineo, Davide

AU - Messinger, Shari

AU - Blomberg, Bonnie B.

AU - Hernandez, Ana

AU - Meneghini, Luigi F.

AU - Allende, Gloria

AU - Skyler, Jay S.

AU - Alejandro, Rodolfo

AU - Pugliese, Alberto

AU - Kenyon, Norma S.

N1 - Funding Information: This work was supported by the Diabetes Research Institute Foundation, Hollywood, FL, USA and the following grants: National Institutes of Health ( U01 DK061041 , U01 DK061037 , UO1 DK070460 ).

PY - 2011/6

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N2 - There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-αalpha;, IFN-γgamma;, Foxp3, IL-10, TGF-βbeta;, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-γ, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG and up-regulation of IFN-γ with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease.

AB - There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-αalpha;, IFN-γgamma;, Foxp3, IL-10, TGF-βbeta;, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-γ, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG and up-regulation of IFN-γ with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease.

KW - Age

KW - Biomarkers

KW - Cytokines

KW - Cytotoxic lymphocyte genes

KW - Gene expression

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Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes

Abstract

Keywords

ASJC Scopus subject areas

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