Immune protection and control of inflammatory tissuenecrosis by γδ T cells

Yang Xin Fu, Christina Ellis Roark, Katherine Kelly, Douglas Drevets, Priscilla Campbell, Rebecca O'Brien, Willi Born

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Host defenses against experimental listeriosis in mice involve neutrophils, macrophages, NK cells, and αβ T cells. Recently γδ cells have also been implicated in antilisterial resistance. However, their specific role has remained unclear. Here we show that efficient resistance to infection by this bacterium depends on the functions of both αβ and γδ cells in both primary and secondary responses. We also present evidence that these functions are complementary. In the livers of αβ T cell-depleted mice, bacteria grow to large numbers within hepatocytes but are infrequently found extracellularly. Granulomatous lesions are more frequent and somewhat larger than in normal controls, but remain focal. Neutrophils are absent from liver lesions in these mice. In contrast, the livers of γδ T cell-depleted mice contain many extracellular bacteria, but do not show hepatocytes containing large numbers of Listeria. Liver lesions in γδ cell-depleted mice are far more extensive than in normal controls or in αβ cell-depleted mice, and contain large numbers of neutrophils. Particularly in secondary listeriosis, γδ T cell-depleted mice show vast coalescent areas of necrotic liver parenchyma within 48 h after infection. Because the bacterial numbers in γδ cell-depleted mice remain lower than in γδ cell-depleted mice, increased mortality in the former may be in part caused by liver failure. We conclude that γδ cells are required to control inflammatory reactivity and to prevent excessive liver damage during the immune response to Listeria monocytogenes.

Original languageEnglish (US)
Pages (from-to)3101-3115
Number of pages15
JournalJournal of Immunology
Volume153
Issue number7
StatePublished - Oct 1 1994

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T-Lymphocytes
Liver
Listeriosis
Neutrophils
Bacteria
Hepatocytes
Listeria
Liver Failure
Listeria monocytogenes
Infection
Natural Killer Cells
Cell Count
Macrophages
Mortality

ASJC Scopus subject areas

  • Immunology

Cite this

Fu, Y. X., Roark, C. E., Kelly, K., Drevets, D., Campbell, P., O'Brien, R., & Born, W. (1994). Immune protection and control of inflammatory tissuenecrosis by γδ T cells. Journal of Immunology, 153(7), 3101-3115.

Immune protection and control of inflammatory tissuenecrosis by γδ T cells. / Fu, Yang Xin; Roark, Christina Ellis; Kelly, Katherine; Drevets, Douglas; Campbell, Priscilla; O'Brien, Rebecca; Born, Willi.

In: Journal of Immunology, Vol. 153, No. 7, 01.10.1994, p. 3101-3115.

Research output: Contribution to journalArticle

Fu, YX, Roark, CE, Kelly, K, Drevets, D, Campbell, P, O'Brien, R & Born, W 1994, 'Immune protection and control of inflammatory tissuenecrosis by γδ T cells', Journal of Immunology, vol. 153, no. 7, pp. 3101-3115.
Fu YX, Roark CE, Kelly K, Drevets D, Campbell P, O'Brien R et al. Immune protection and control of inflammatory tissuenecrosis by γδ T cells. Journal of Immunology. 1994 Oct 1;153(7):3101-3115.
Fu, Yang Xin ; Roark, Christina Ellis ; Kelly, Katherine ; Drevets, Douglas ; Campbell, Priscilla ; O'Brien, Rebecca ; Born, Willi. / Immune protection and control of inflammatory tissuenecrosis by γδ T cells. In: Journal of Immunology. 1994 ; Vol. 153, No. 7. pp. 3101-3115.
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abstract = "Host defenses against experimental listeriosis in mice involve neutrophils, macrophages, NK cells, and αβ T cells. Recently γδ cells have also been implicated in antilisterial resistance. However, their specific role has remained unclear. Here we show that efficient resistance to infection by this bacterium depends on the functions of both αβ and γδ cells in both primary and secondary responses. We also present evidence that these functions are complementary. In the livers of αβ T cell-depleted mice, bacteria grow to large numbers within hepatocytes but are infrequently found extracellularly. Granulomatous lesions are more frequent and somewhat larger than in normal controls, but remain focal. Neutrophils are absent from liver lesions in these mice. In contrast, the livers of γδ T cell-depleted mice contain many extracellular bacteria, but do not show hepatocytes containing large numbers of Listeria. Liver lesions in γδ cell-depleted mice are far more extensive than in normal controls or in αβ cell-depleted mice, and contain large numbers of neutrophils. Particularly in secondary listeriosis, γδ T cell-depleted mice show vast coalescent areas of necrotic liver parenchyma within 48 h after infection. Because the bacterial numbers in γδ cell-depleted mice remain lower than in γδ cell-depleted mice, increased mortality in the former may be in part caused by liver failure. We conclude that γδ cells are required to control inflammatory reactivity and to prevent excessive liver damage during the immune response to Listeria monocytogenes.",
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