Immune rejection of metastases arising from intraocular tumors in mice

J. Y. Niederkorn, T. L. Knisely, E. Mayhew

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The role of the immune response in the elimination of spontaneous metastases arising from intraocular tumors was examined in a syngeneic intraocular murine tumor model. P91 mastocytoma (DBA/2 origin) expresses strong tumor-specific transplantation antigens and grows transiently in the eyes of syngeneic hosts before undergoing spontaneous rejection. An organ culture technique was used to detect spontaneous metastases in the lungs, spleens, brains, and thymus of intraocular tumor-bearing mice. Metastatic tumor cells were detected in all organs of immunodeficient mice (i.e., athymic, nude, or x-irradiated DBA/2 mice) within 14 days of intraocular transplantation, and grew progressively thereafter. By contrast, metastatic tumors were rejected in 100% of the immunocompetent DBA/2 mice examined on day 15. Timed enucleation experiments demonstrated that the immune rejection of disseminated tumor cells occurred within 24-48 hr of their arrival at the various organs. The immune rejection of spontaneous metastases could be adoptively transferred to immunodeficient tumor-bearing mice using spleen cells, but not immune serum, from intraocular tumor-bearing immunocompetent donors. Selective cell depletion experiments revealed that the immune spleen cell effecting immunity was an Lyt 1+, 2+ T cell. The results indicate that the immune rejection of the spontaneous metastases arising from primary intraocular tumors is a T cell-dependent, radiosensitive process that rapidly eliminates metastases within the lungs, brain, thymus, and the spleen of the immunocompetent host.

Original languageEnglish (US)
Pages (from-to)1355-1361
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume27
Issue number9
StatePublished - 1986

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Neoplasm Metastasis
Neoplasms
Spleen
Inbred DBA Mouse
Mastocytoma
Thymus Neoplasms
T-Lymphocytes
Lung
Organ Culture Techniques
Brain
Nude Mice
Thymus Gland
Immune Sera
Immunity
Transplantation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Immune rejection of metastases arising from intraocular tumors in mice. / Niederkorn, J. Y.; Knisely, T. L.; Mayhew, E.

In: Investigative Ophthalmology and Visual Science, Vol. 27, No. 9, 1986, p. 1355-1361.

Research output: Contribution to journalArticle

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