Immuno-, lectin-, and enzymehistochemical characterization of human bone marrow endotheliumee

L. C. Masek, J. W. Sweetenham, J. M.A. Whitehouse, U. Schumacher

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

'Homing' of hematopoietic progenitor cells to the bone marrow occurs during the clinical practice of bone marrow transplantation. Its mechanism is unknown, although adhesive interactions between hematopoietic cells and sinusoidal endothelium in the bone marrow may be implicated. Studies of human bone marrow endothelial cells have previously been limited by the lack of markers for these cells. In this report, we describe positive staining of bone marrow endothelial cells from human bone marrow trephine biopsies with antibody to factor VIII-related antigen (FVIIIR-Ag) (Dako, High Wycombe, UK), the plant lectin Ulex europaeus agglutinin-I (UEA-I), and two mouse monoclonal antibodies, BMA120 and QBEND/10. In addition, alkaline phosphatase could be demonstrated in the majority of marrow endothelial cells using a novel enzyme histochemical technique. These studies defined the marker profile of human marrow endothelium. The results of this study will facilitate the isolation and culture of human marrow endothelial cells for in vitro studies of their roles in hematopoietic stem cell homing.

Original languageEnglish (US)
Pages (from-to)1203-1209
Number of pages7
JournalExperimental Hematology
Volume22
Issue number12
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Lectins
Bone Marrow
Endothelial Cells
Hematopoietic Stem Cells
Bone Marrow Cells
Endothelium
Plant Lectins
Ulex
Agglutinins
von Willebrand Factor
Bone Marrow Transplantation
Adhesives
Alkaline Phosphatase
Monoclonal Antibodies
Staining and Labeling
Biopsy
Antibodies
Enzymes

Keywords

  • Alkaline phosphatase
  • Bone marrow endothelium
  • Factor VIII-related antigen
  • Lectins
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Cite this

Immuno-, lectin-, and enzymehistochemical characterization of human bone marrow endotheliumee. / Masek, L. C.; Sweetenham, J. W.; Whitehouse, J. M.A.; Schumacher, U.

In: Experimental Hematology, Vol. 22, No. 12, 01.01.1994, p. 1203-1209.

Research output: Contribution to journalArticle

Masek, L. C. ; Sweetenham, J. W. ; Whitehouse, J. M.A. ; Schumacher, U. / Immuno-, lectin-, and enzymehistochemical characterization of human bone marrow endotheliumee. In: Experimental Hematology. 1994 ; Vol. 22, No. 12. pp. 1203-1209.
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