Immunoconjugates containing ricin a chain and either human anti-gp41 or CD4 kill H9 cells infected with different isolates of HIV, but do not inhibit normal t or b cell function

M. A. Till, V. Ghetie, R. D. May, P. C. Auerbach, S. Zolla-Pazner, M. K. Gorny, T. Gregory, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have previously reported that immunoconjugates composed of deglycosylated ricin A chain coupled to either recombinant (r) CD4 or two different monoclonal human anti-gp41 antibodies (rCD4-dgA and anti-gp41-dgA, respectively) are specifically toxic to HIV-infected lines of human T cells (Hg) and monocytes (U937). In order to further evaluate these immunoconjugates as potential therapeutic reagents for killing HIV-infected cells, H9 cells infected with five different isolates of HIV were used as target cells in vitro. All three HIV-specific immunoconjugates were toxic to H9 cells infected with each HIV isolate, but were virtually nontoxic to uninfected cells. Chlorouquine markedly potentiated the specific toxicity of all three conjugates, particularly the anti-gp41-dgAs. None of the conjugates affected the ability of normal peripheral blood B cells to respond to mitogen or the ability of normal T cells to respond to alloantigens.

Original languageEnglish (US)
Pages (from-to)609-614
Number of pages6
JournalJournal of Acquired Immune Deficiency Syndromes
Volume3
Issue number6
StatePublished - 1990

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Immunoconjugates
Ricin
HIV
Poisons
T-Lymphocytes
Isoantigens
Mitogens
Monocytes
Anti-Idiotypic Antibodies
Blood Cells
B-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Virology

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Immunoconjugates containing ricin a chain and either human anti-gp41 or CD4 kill H9 cells infected with different isolates of HIV, but do not inhibit normal t or b cell function. / Till, M. A.; Ghetie, V.; May, R. D.; Auerbach, P. C.; Zolla-Pazner, S.; Gorny, M. K.; Gregory, T.; Uhr, J. W.; Vitetta, E. S.

In: Journal of Acquired Immune Deficiency Syndromes, Vol. 3, No. 6, 1990, p. 609-614.

Research output: Contribution to journalArticle

Till, M. A. ; Ghetie, V. ; May, R. D. ; Auerbach, P. C. ; Zolla-Pazner, S. ; Gorny, M. K. ; Gregory, T. ; Uhr, J. W. ; Vitetta, E. S. / Immunoconjugates containing ricin a chain and either human anti-gp41 or CD4 kill H9 cells infected with different isolates of HIV, but do not inhibit normal t or b cell function. In: Journal of Acquired Immune Deficiency Syndromes. 1990 ; Vol. 3, No. 6. pp. 609-614.
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abstract = "We have previously reported that immunoconjugates composed of deglycosylated ricin A chain coupled to either recombinant (r) CD4 or two different monoclonal human anti-gp41 antibodies (rCD4-dgA and anti-gp41-dgA, respectively) are specifically toxic to HIV-infected lines of human T cells (Hg) and monocytes (U937). In order to further evaluate these immunoconjugates as potential therapeutic reagents for killing HIV-infected cells, H9 cells infected with five different isolates of HIV were used as target cells in vitro. All three HIV-specific immunoconjugates were toxic to H9 cells infected with each HIV isolate, but were virtually nontoxic to uninfected cells. Chlorouquine markedly potentiated the specific toxicity of all three conjugates, particularly the anti-gp41-dgAs. None of the conjugates affected the ability of normal peripheral blood B cells to respond to mitogen or the ability of normal T cells to respond to alloantigens.",
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AU - Till, M. A.

AU - Ghetie, V.

AU - May, R. D.

AU - Auerbach, P. C.

AU - Zolla-Pazner, S.

AU - Gorny, M. K.

AU - Gregory, T.

AU - Uhr, J. W.

AU - Vitetta, E. S.

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AB - We have previously reported that immunoconjugates composed of deglycosylated ricin A chain coupled to either recombinant (r) CD4 or two different monoclonal human anti-gp41 antibodies (rCD4-dgA and anti-gp41-dgA, respectively) are specifically toxic to HIV-infected lines of human T cells (Hg) and monocytes (U937). In order to further evaluate these immunoconjugates as potential therapeutic reagents for killing HIV-infected cells, H9 cells infected with five different isolates of HIV were used as target cells in vitro. All three HIV-specific immunoconjugates were toxic to H9 cells infected with each HIV isolate, but were virtually nontoxic to uninfected cells. Chlorouquine markedly potentiated the specific toxicity of all three conjugates, particularly the anti-gp41-dgAs. None of the conjugates affected the ability of normal peripheral blood B cells to respond to mitogen or the ability of normal T cells to respond to alloantigens.

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