Immunocytochemical evidence for the acidic nature of peroxisomes in methylotrophic yeasts

Hans R. Waterham, Ineke Keizer-Gunnink, Joel M. Goodman, Wim Harder, Marten Veenhuis

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

The possible acidic nature of the peroxisomal matrix present in intact yeast cells was studied immunocytochemically, using the weak base DAMP as a probe. Spheroplasts of methanol-grown Candida boidinii and Hansenula polymorpha were regenerated and incubated with DAMP. After immunogold labelling, using antibodies against DAMP, a specific accumulation of gold particles was observed on the peroxisomal profiles. This labelling was absent in controls, performed in the presence of ionophores or chloroquine. These results support earlier observations, that in intact cells a pH-gradient exists across the peroxisomal membrane. Experiments, carried out on osmotically swollen spheroplasts indicated that maintenance of this pH-gradient is strongly related to the cell's integrity.

Original languageEnglish (US)
Pages (from-to)17-19
Number of pages3
JournalFEBS Letters
Volume262
Issue number1
DOIs
StatePublished - Mar 12 1990

Keywords

  • (Candida boidinii
  • Hansenula polymorpha)
  • Immunocytochemistry
  • Peroxisome
  • Proton gradient

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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