Immunohistochemical staining of cholinergic neurons in the human brain using a polyclonal antibody to human choline acetyltransferase

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Abstract

Antibodies against human placental choline acetyltransferase (ChAT) were used to immunohistochemically stain cholinergic neurons in the neostriatum and nucleus basalis of Meynert in human brain. Cells in both regions were intensely stained as were nerve fibers. Comparable cells were stained in these same brain regions in the rat. This anti-human ChAT antibody will enable the further detailed characterization of cholinergic neurons in the human brain in both health and disease.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalNeuroscience Letters
Volume61
Issue number1-2
DOIs
StatePublished - Oct 24 1985

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Cholinergic Neurons
Choline O-Acetyltransferase
Staining and Labeling
Antibodies
Brain
Basal Nucleus of Meynert
Neostriatum
Nerve Fibers
Coloring Agents
Health

Keywords

  • choline acetyltransferase
  • human brain
  • immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "Antibodies against human placental choline acetyltransferase (ChAT) were used to immunohistochemically stain cholinergic neurons in the neostriatum and nucleus basalis of Meynert in human brain. Cells in both regions were intensely stained as were nerve fibers. Comparable cells were stained in these same brain regions in the rat. This anti-human ChAT antibody will enable the further detailed characterization of cholinergic neurons in the human brain in both health and disease.",
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AB - Antibodies against human placental choline acetyltransferase (ChAT) were used to immunohistochemically stain cholinergic neurons in the neostriatum and nucleus basalis of Meynert in human brain. Cells in both regions were intensely stained as were nerve fibers. Comparable cells were stained in these same brain regions in the rat. This anti-human ChAT antibody will enable the further detailed characterization of cholinergic neurons in the human brain in both health and disease.

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KW - immunohistochemistry

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