TY - JOUR
T1 - Immunohistochemical studies of steroidogenic enzymes (aromatase, 17α-hydroxylase and cholesterol side-chain cleavage cytochromes P-450) in sex cord-stromal tumors of the ovary
AU - Sasano, Hironobu
AU - Okamoto, Mitsuhiro
AU - Mason, J. Ian
AU - Simpson, Evan R.
AU - Mendelson, Carrol R.
AU - Sasano, Nobuaki
AU - Silverberg, Steven G.
N1 - Funding Information:
From the Department of Pathology, Tohoku University School of Medicine, Sendai, Japan; the Department of Pathology, The George Washington University Medical Center, Washington, DC; the Department of Molecular and Physiological Chemistry, Osaka University, Osaka, Japan; and the Departments of Biochemistry and Obstetrics and Gynecology, Cecil H. and Ida Green Center for Reproductive Biology, The University of Texas Southwestern Medical Center at Dallas. Accepted for publication December 6, 1988. Supported in part by United States Public Health Service grants no. AG00306, AM31206, and CA30253. Presented in part at the XVII International Congress of the International Academy of Pathology, Dublin, Ireland (September 1988). Key words: cytochromes P-450, ovarian neoplasms, steroidogenesis. Address correspondence and reprint requests to Steven G. Silverberg, MD, Department of Pathology, The George Washington University Medical Center, 2300 Eye St, NW, Washington, DC 20037. 0 1989 by W.B. Saunders Company. 0046-B 177/89/2005-0010$5.00/O
PY - 1989/5
Y1 - 1989/5
N2 - Aromatase, 17α-hydroxylase, and cholesterol side-chain cleavage P-450 cytochromes (P-450arom, P-45017α, and P-450scc, respectively) were immunohistochemically localized in nine granulosa cell tumors, 15 thecomas, ten Sertoli-Leydig cell tumors, two steroid cell tumors, five fibromas, and five sclerosing stromal tumors. In the thecomas, P-450scc and P-45017α were positive in luteinized theca cells and in cells with vacuolated cytoplasm, while P-450arom was not observed. In the steroid cell tumors, all the P-450 cytochromes were intensely stained. P-450scc and P-45017α were present in cells with vacuolated cytoplasm in two cases of sclerosing stromal tumor. P-450arom was weakly demonstrated in one of the granulosa cell tumors. P-45017α, P-450scc, and P-450arom were all faintly stained in the Sertoli-Leydig cell tumors. No P-450 cytochrome immunoreactivity was observed in any fibroma.
AB - Aromatase, 17α-hydroxylase, and cholesterol side-chain cleavage P-450 cytochromes (P-450arom, P-45017α, and P-450scc, respectively) were immunohistochemically localized in nine granulosa cell tumors, 15 thecomas, ten Sertoli-Leydig cell tumors, two steroid cell tumors, five fibromas, and five sclerosing stromal tumors. In the thecomas, P-450scc and P-45017α were positive in luteinized theca cells and in cells with vacuolated cytoplasm, while P-450arom was not observed. In the steroid cell tumors, all the P-450 cytochromes were intensely stained. P-450scc and P-45017α were present in cells with vacuolated cytoplasm in two cases of sclerosing stromal tumor. P-450arom was weakly demonstrated in one of the granulosa cell tumors. P-45017α, P-450scc, and P-450arom were all faintly stained in the Sertoli-Leydig cell tumors. No P-450 cytochrome immunoreactivity was observed in any fibroma.
KW - cytochromes P-450
KW - ovarian neoplasms
KW - steroidogenesis
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U2 - 10.1016/0046-8177(89)90010-5
DO - 10.1016/0046-8177(89)90010-5
M3 - Article
C2 - 2540088
AN - SCOPUS:0024398789
SN - 0046-8177
VL - 20
SP - 452
EP - 457
JO - Human Pathology
JF - Human Pathology
IS - 5
ER -