Immunohistochemical studies of steroidogenic enzymes (aromatase, 17α-hydroxylase and cholesterol side-chain cleavage cytochromes P-450) in sex cord-stromal tumors of the ovary

Hironobu Sasano, Mitsuhiro Okamoto, J. Ian Mason, Evan R. Simpson, Carrol R. Mendelson, Nobuaki Sasano, Steven G. Silverberg

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Abstract

Aromatase, 17α-hydroxylase, and cholesterol side-chain cleavage P-450 cytochromes (P-450arom, P-45017α, and P-450scc, respectively) were immunohistochemically localized in nine granulosa cell tumors, 15 thecomas, ten Sertoli-Leydig cell tumors, two steroid cell tumors, five fibromas, and five sclerosing stromal tumors. In the thecomas, P-450scc and P-45017α were positive in luteinized theca cells and in cells with vacuolated cytoplasm, while P-450arom was not observed. In the steroid cell tumors, all the P-450 cytochromes were intensely stained. P-450scc and P-45017α were present in cells with vacuolated cytoplasm in two cases of sclerosing stromal tumor. P-450arom was weakly demonstrated in one of the granulosa cell tumors. P-45017α, P-450scc, and P-450arom were all faintly stained in the Sertoli-Leydig cell tumors. No P-450 cytochrome immunoreactivity was observed in any fibroma.

Original languageEnglish (US)
Pages (from-to)452-457
Number of pages6
JournalHuman Pathology
Volume20
Issue number5
DOIs
StatePublished - 1989

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Sex Cord-Gonadal Stromal Tumors
Aromatase
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Ovary
Thecoma
Sertoli-Leydig Cell Tumor
Cholesterol
Granulosa Cell Tumor
Fibroma
Enzymes
Neoplasms
Cytoplasm
Steroids
Theca Cells

Keywords

  • cytochromes P-450
  • ovarian neoplasms
  • steroidogenesis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistochemical studies of steroidogenic enzymes (aromatase, 17α-hydroxylase and cholesterol side-chain cleavage cytochromes P-450) in sex cord-stromal tumors of the ovary. / Sasano, Hironobu; Okamoto, Mitsuhiro; Mason, J. Ian; Simpson, Evan R.; Mendelson, Carrol R.; Sasano, Nobuaki; Silverberg, Steven G.

In: Human Pathology, Vol. 20, No. 5, 1989, p. 452-457.

Research output: Contribution to journalArticle

Sasano, Hironobu ; Okamoto, Mitsuhiro ; Mason, J. Ian ; Simpson, Evan R. ; Mendelson, Carrol R. ; Sasano, Nobuaki ; Silverberg, Steven G. / Immunohistochemical studies of steroidogenic enzymes (aromatase, 17α-hydroxylase and cholesterol side-chain cleavage cytochromes P-450) in sex cord-stromal tumors of the ovary. In: Human Pathology. 1989 ; Vol. 20, No. 5. pp. 452-457.
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abstract = "Aromatase, 17α-hydroxylase, and cholesterol side-chain cleavage P-450 cytochromes (P-450arom, P-45017α, and P-450scc, respectively) were immunohistochemically localized in nine granulosa cell tumors, 15 thecomas, ten Sertoli-Leydig cell tumors, two steroid cell tumors, five fibromas, and five sclerosing stromal tumors. In the thecomas, P-450scc and P-45017α were positive in luteinized theca cells and in cells with vacuolated cytoplasm, while P-450arom was not observed. In the steroid cell tumors, all the P-450 cytochromes were intensely stained. P-450scc and P-45017α were present in cells with vacuolated cytoplasm in two cases of sclerosing stromal tumor. P-450arom was weakly demonstrated in one of the granulosa cell tumors. P-45017α, P-450scc, and P-450arom were all faintly stained in the Sertoli-Leydig cell tumors. No P-450 cytochrome immunoreactivity was observed in any fibroma.",
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