Immunohistochemical studies of steroidogenic enzymes (aromatase, 17α-hydroxylase and cholesterol side-chain cleavage cytochromes P-450) in sex cord-stromal tumors of the ovary

Hironobu Sasano, Mitsuhiro Okamoto, J. Ian Mason, Evan R. Simpson, Carrol R. Mendelson, Nobuaki Sasano, Steven G. Silverberg

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Aromatase, 17α-hydroxylase, and cholesterol side-chain cleavage P-450 cytochromes (P-450arom, P-45017α, and P-450scc, respectively) were immunohistochemically localized in nine granulosa cell tumors, 15 thecomas, ten Sertoli-Leydig cell tumors, two steroid cell tumors, five fibromas, and five sclerosing stromal tumors. In the thecomas, P-450scc and P-45017α were positive in luteinized theca cells and in cells with vacuolated cytoplasm, while P-450arom was not observed. In the steroid cell tumors, all the P-450 cytochromes were intensely stained. P-450scc and P-45017α were present in cells with vacuolated cytoplasm in two cases of sclerosing stromal tumor. P-450arom was weakly demonstrated in one of the granulosa cell tumors. P-45017α, P-450scc, and P-450arom were all faintly stained in the Sertoli-Leydig cell tumors. No P-450 cytochrome immunoreactivity was observed in any fibroma.

Original languageEnglish (US)
Pages (from-to)452-457
Number of pages6
JournalHuman Pathology
Issue number5
Publication statusPublished - 1989



  • cytochromes P-450
  • ovarian neoplasms
  • steroidogenesis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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