Immunohistochemistry of cytochrome P-450 21-hydroxylase: Microscopic examination of the enzyme in the bovine adrenal cortex and kidney

H. Sasano, P. C. White, M. I. New, N. Sasano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cytochrome P-450 specific for steroid 21-hydroxylase (P-450(C21)) localized in bovine adrenal cortex and kidney was immunocytochemically observed by the peroxidase-antiperoxidase method using a specific antibody. P-450(C21) was present in all three zones of the adrenal cortex. Immunoreactivity for P-450(C21) was intense in the zona glomerulosa and inner reticularis and faint in the area between the zona glomerulosa and outer fasciculata, probably representing the zona intermedia. The positive stain was only observed in parenchymal cells. The immunoreactivity varied within each zone, especially in the zona reticularis. In the kidney, immunoreactivity for P-450(C21) was exclusively localized in the distal and cortical and medullary collecting tubules. This corresponds to the site of mineralocorticoid action in the kidney. No immunoreactivity was observed in the liver and aorta.

Original languageEnglish (US)
Pages (from-to)291-295
Number of pages5
JournalEndocrinology
Volume122
Issue number1
StatePublished - 1988

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Steroid 21-Hydroxylase
Adrenal Cortex
Immunohistochemistry
Zona Glomerulosa
Kidney
Enzymes
Zona Reticularis
Mineralocorticoids
Herpes Zoster
Cytochrome P-450 Enzyme System
Peroxidase
Aorta
Coloring Agents
Antibodies
Liver

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Immunohistochemistry of cytochrome P-450 21-hydroxylase : Microscopic examination of the enzyme in the bovine adrenal cortex and kidney. / Sasano, H.; White, P. C.; New, M. I.; Sasano, N.

In: Endocrinology, Vol. 122, No. 1, 1988, p. 291-295.

Research output: Contribution to journalArticle

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