Immunolocalization of the Nuk receptor tyrosine kinase suggests roles in segmental patterning of the brain and axonogenesis

Mark Henkemeyer, Luc E M Marengere, Jane McGlade, Jean Paul Olivier, Ronald A. Conlon, Douglas P. Holmyard, Kenneth Letwin, Tony Pawson

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Abstract

Neural kinase (Nuk) encodes a murine receptor-like tyrosine kinase belonging to the Eph/Elk/Eck family. Protein localization studies indicate that during early embryogenesis Nuk is confined to the developing nervous system, where it marks segments along the axis of the neural tube in the hindbrain (rhombomeres r2, r3 and r5) and specific morphological bulges of the midbrain and forebrain. Subcellular localization of Nuk indicates that this receptor is concentrated at sites of cell-cell contact, often involving migrating neuronal cells or their extensions. Most notably, high levels of Nuk protein are found within initial axon outgrowths and associated nerve fibers. The axonal localization of Nuk is transient and is not detected after migrations have ceased, suggesting a role for this tyrosine kinase during the early pathfinding and/or fasciculation stages of axonogenesis. The subcellular localization of Nuk, as well as the presence of fibronectin type III and immunoglobulin-like adhesive domains on the extracellular region, suggest this receptor tyrosine kinase may function to regulate specific cell-cell interactions during early development of the murine nervous system.

Original languageEnglish (US)
Pages (from-to)1001-1014
Number of pages14
JournalOncogene
Volume9
Issue number4
StatePublished - Apr 1994

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Sensory Receptor Cells
Protein-Tyrosine Kinases
Phosphotransferases
Brain
Receptor Protein-Tyrosine Kinases
Nervous System
Fasciculation
Rhombencephalon
Neural Tube
Prosencephalon
Mesencephalon
Nerve Fibers
Fibronectins
Cell Communication
Adhesives
Protein Kinases
Embryonic Development
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Henkemeyer, M., Marengere, L. E. M., McGlade, J., Olivier, J. P., Conlon, R. A., Holmyard, D. P., ... Pawson, T. (1994). Immunolocalization of the Nuk receptor tyrosine kinase suggests roles in segmental patterning of the brain and axonogenesis. Oncogene, 9(4), 1001-1014.

Immunolocalization of the Nuk receptor tyrosine kinase suggests roles in segmental patterning of the brain and axonogenesis. / Henkemeyer, Mark; Marengere, Luc E M; McGlade, Jane; Olivier, Jean Paul; Conlon, Ronald A.; Holmyard, Douglas P.; Letwin, Kenneth; Pawson, Tony.

In: Oncogene, Vol. 9, No. 4, 04.1994, p. 1001-1014.

Research output: Contribution to journalArticle

Henkemeyer, M, Marengere, LEM, McGlade, J, Olivier, JP, Conlon, RA, Holmyard, DP, Letwin, K & Pawson, T 1994, 'Immunolocalization of the Nuk receptor tyrosine kinase suggests roles in segmental patterning of the brain and axonogenesis', Oncogene, vol. 9, no. 4, pp. 1001-1014.
Henkemeyer M, Marengere LEM, McGlade J, Olivier JP, Conlon RA, Holmyard DP et al. Immunolocalization of the Nuk receptor tyrosine kinase suggests roles in segmental patterning of the brain and axonogenesis. Oncogene. 1994 Apr;9(4):1001-1014.
Henkemeyer, Mark ; Marengere, Luc E M ; McGlade, Jane ; Olivier, Jean Paul ; Conlon, Ronald A. ; Holmyard, Douglas P. ; Letwin, Kenneth ; Pawson, Tony. / Immunolocalization of the Nuk receptor tyrosine kinase suggests roles in segmental patterning of the brain and axonogenesis. In: Oncogene. 1994 ; Vol. 9, No. 4. pp. 1001-1014.
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