Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system

Yu Yen Huang, Kazunori Hoshino, Peng Chen, Chun Hsien Wu, Nancy Lane, Michael Huebschman, Huaying Liu, Konstantin Sokolov, Jonathan W. Uhr, Eugene P. Frenkel, John X J Zhang

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Combining the power of immunomagnetic assay and microfluidic microchip operations, we successfully detected rare CTCs from clinical blood samples. The microfluidic system is operated in a flip-flop mode, where a computer-controlled rotational holder with an array of microfluidic chips inverts the microchannels. We have demonstrated both theoretically and experimentally that the direction of red blood cell (RBC) sedimentation with regards to the magnetic force required for cell separation is important for capture efficiency, throughput, and purity. The flip-flop operation reduces the stagnation of RBCs and non-specific binding on the capture surface by alternating the direction of the magnetic field with respect to gravity. The developed immunomagnetic microchip-based screening system exhibits high capture rates (more than 90%) for SkBr3, PC3, and Colo205 cell lines in spiked screening experiments and successfully isolates CTCs from patient blood samples. The proposed motion controlled microchip-based immunomagnetic system shows great promise as a clinical tool for cancer diagnosis and prognosis.

Original languageEnglish (US)
Pages (from-to)673-681
Number of pages9
JournalBiomedical Microdevices
Volume15
Issue number4
DOIs
StatePublished - Aug 1 2013

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Keywords

  • Capture efficiency
  • Circulating tumor cells (CTCs)
  • Fluorescent imaging
  • Gravity
  • Immunomagnetic assay
  • Microfluidic chip
  • Purity

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

Cite this

Huang, Y. Y., Hoshino, K., Chen, P., Wu, C. H., Lane, N., Huebschman, M., Liu, H., Sokolov, K., Uhr, J. W., Frenkel, E. P., & Zhang, J. X. J. (2013). Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system. Biomedical Microdevices, 15(4), 673-681. https://doi.org/10.1007/s10544-012-9718-8