Immunophenotyping by multiparameter flow cytometry

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Multiparameter flow cytometry has become an indispensable tool for the diagnosis and classification of acute myeloid leukemia (AML). The basic method relies on the unique ability to detect immunophenotypic abnormalities on discrete subpopulations. The primary roles in the initial assessment of AML are to determine the immaturity of the leukemic population, define the lineage and the immunophenotypic aberrancies in blasts, and identify characteristic immunophenotypic features to predict important recurrent cytogenetic and genetic abnormalities and prognosis. The established immunophenotypic profile, a baseline “fingerprint,” is used for follow-up assessment of residual disease. This chapter provides an overview of procedures for specimen processing, staining, and immunophenotyping of AML and describes our strategy for data analysis supplemented with illustrative case examples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages51-73
Number of pages23
Volume1633
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1633
ISSN (Print)1064-3745

Fingerprint

Immunophenotyping
Acute Myeloid Leukemia
Flow Cytometry
Dermatoglyphics
Chromosome Aberrations
Staining and Labeling
Population

Keywords

  • Acute myeloid leukemia
  • Cytogenetics
  • Flow cytometry
  • Immunophenotyping
  • Residual disease

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chen, W., & Luu, H. S. (2017). Immunophenotyping by multiparameter flow cytometry. In Methods in Molecular Biology (Vol. 1633, pp. 51-73). (Methods in Molecular Biology; Vol. 1633). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7142-8_4

Immunophenotyping by multiparameter flow cytometry. / Chen, Weina; Luu, Hung S.

Methods in Molecular Biology. Vol. 1633 Humana Press Inc., 2017. p. 51-73 (Methods in Molecular Biology; Vol. 1633).

Research output: Chapter in Book/Report/Conference proceedingChapter

Chen, W & Luu, HS 2017, Immunophenotyping by multiparameter flow cytometry. in Methods in Molecular Biology. vol. 1633, Methods in Molecular Biology, vol. 1633, Humana Press Inc., pp. 51-73. https://doi.org/10.1007/978-1-4939-7142-8_4
Chen W, Luu HS. Immunophenotyping by multiparameter flow cytometry. In Methods in Molecular Biology. Vol. 1633. Humana Press Inc. 2017. p. 51-73. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7142-8_4
Chen, Weina ; Luu, Hung S. / Immunophenotyping by multiparameter flow cytometry. Methods in Molecular Biology. Vol. 1633 Humana Press Inc., 2017. pp. 51-73 (Methods in Molecular Biology).
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