Impact of MR on mature adipocytes in high-fat/high-sucrose diet-induced obesity

Tomoaki Hayakawa, Tomomi Minemura, Toshiharu Onodera, Jihoon Shin, Yosuke Okuno, Atsunori Fukuhara, Michio Otsuki, Iichiro Shimomura

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Active glucocorticoid levels are elevated in the adipose tissue of obesity due to the enzyme 11 beta-hydroxysteroid dehydrogenase type 1. Glucocorticoids can bind and activate both glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), and pharmacological blockades of MR prevent high-fat diet-induced obesity and glucose intolerance. To determine the significance of MR in adipocytes, we generated adipocytespecific MR-knockout mice (AdipoMR-KO) and fed them high-fat/high-sucrose diet. We found that adipocyte-specific deletion of MR did not affect the body weight, fat weight, glucose tolerance or insulin sensitivity. While liver weight was slightly reduced in AdipoMR-KO, there were no significant differences in the mRNA expression levels of genes associated with lipogenesis, lipolysis, adipocytokines and oxidative stress in adipose tissues between the control and AdipoMR-KO mice. The results indicated that MR in mature adipocytes plays a minor role in the regulation of insulin resistance and inflammation in high-fat/high-sucrose diet-induced obese mice.

Original languageEnglish (US)
Pages (from-to)63-72
Number of pages10
JournalJournal of Endocrinology
Volume239
Issue number1
DOIs
StatePublished - Oct 1 2018

Keywords

  • Adipocyte
  • Insulin resistance
  • Mineralocorticoid
  • Obesity
  • Receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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