Impact of the loss of caveolin-1 on lung mass and cholesterol metabolism in mice with and without the lysosomal cholesterol transporter, Niemann-Pick type C1

Dorothy I. Mundy, Adam M. Lopez, Kenneth S. Posey, Jen Chieh Chuang, Charina M. Ramirez, Philipp E. Scherer, Stephen D. Turley

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Abstract

Caveolin-1 (Cav-1) is a major structural protein in caveolae in the plasma membranes of many cell types, particularly endothelial cells and adipocytes. Loss of Cav-1 function has been implicated in multiple diseases affecting the cardiopulmonary and central nervous systems, as well as in specific aspects of sterol and lipid metabolism in the liver and intestine. Lungs contain an exceptionally high level of Cav-1. Parameters of cholesterol metabolism in the lung were measured, initially in Cav-1-deficient mice (Cav-1-/-), and subsequently in Cav-1-/- mice that also lacked the lysosomal cholesterol transporter Niemann-Pick C1 (Npc1) (Cav-1-/-:Npc1 -/-). In 50-day-old Cav-1-/- mice fed a low- or high-cholesterol chow diet, the total cholesterol concentration (mg/g) in the lungs was marginally lower than in the Cav-1+/+ controls, but due to an expansion in their lung mass exceeding 30%, whole-lung cholesterol content (mg/organ) was moderately elevated. Lung mass (g) in the Cav-1 -/-:Npc1-/- mice (0.356 ± 0.022) markedly exceeded that in their Cav-1+/+:Npc1+/+ controls (0.137 ± 0.009), as well as in their Cav-1-/-:Npc1+/+ (0.191 ± 0.013) and Cav-1+/+:Npc1-/- (0.213 ± 0.022) littermates. The corresponding lung total cholesterol contents (mg/organ) in mice of these genotypes were 6.74 ± 0.17, 0.71 ± 0.05, 0.96 ± 0.05 and 3.12 ± 0.43, respectively, with the extra cholesterol in the Cav-1-/-:Npc1-/- and Cav-1+/+:Npc1 -/- mice being nearly all unesterified (UC). The exacerbation of the Npc1 lung phenotype and increase in the UC level in the Cav-1 -/-:Npc1-/- mice imply a regulatory role of Cav-1 in pulmonary cholesterol metabolism when lysosomal sterol transport is disrupted.

Original languageEnglish (US)
Pages (from-to)995-1002
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1841
Issue number7
DOIs
StatePublished - 2014

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Caveolin 1
Cholesterol
Lung
Sterols
Caveolae

Keywords

  • Cellular cholesterol trafficking
  • Cholesterol feeding
  • Cholesterol synthesis
  • Pulmonary dysfunction
  • Relative organ weight

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

@article{1371017613d6457d9753bb9091e02a52,
title = "Impact of the loss of caveolin-1 on lung mass and cholesterol metabolism in mice with and without the lysosomal cholesterol transporter, Niemann-Pick type C1",
abstract = "Caveolin-1 (Cav-1) is a major structural protein in caveolae in the plasma membranes of many cell types, particularly endothelial cells and adipocytes. Loss of Cav-1 function has been implicated in multiple diseases affecting the cardiopulmonary and central nervous systems, as well as in specific aspects of sterol and lipid metabolism in the liver and intestine. Lungs contain an exceptionally high level of Cav-1. Parameters of cholesterol metabolism in the lung were measured, initially in Cav-1-deficient mice (Cav-1-/-), and subsequently in Cav-1-/- mice that also lacked the lysosomal cholesterol transporter Niemann-Pick C1 (Npc1) (Cav-1-/-:Npc1 -/-). In 50-day-old Cav-1-/- mice fed a low- or high-cholesterol chow diet, the total cholesterol concentration (mg/g) in the lungs was marginally lower than in the Cav-1+/+ controls, but due to an expansion in their lung mass exceeding 30{\%}, whole-lung cholesterol content (mg/organ) was moderately elevated. Lung mass (g) in the Cav-1 -/-:Npc1-/- mice (0.356 ± 0.022) markedly exceeded that in their Cav-1+/+:Npc1+/+ controls (0.137 ± 0.009), as well as in their Cav-1-/-:Npc1+/+ (0.191 ± 0.013) and Cav-1+/+:Npc1-/- (0.213 ± 0.022) littermates. The corresponding lung total cholesterol contents (mg/organ) in mice of these genotypes were 6.74 ± 0.17, 0.71 ± 0.05, 0.96 ± 0.05 and 3.12 ± 0.43, respectively, with the extra cholesterol in the Cav-1-/-:Npc1-/- and Cav-1+/+:Npc1 -/- mice being nearly all unesterified (UC). The exacerbation of the Npc1 lung phenotype and increase in the UC level in the Cav-1 -/-:Npc1-/- mice imply a regulatory role of Cav-1 in pulmonary cholesterol metabolism when lysosomal sterol transport is disrupted.",
keywords = "Cellular cholesterol trafficking, Cholesterol feeding, Cholesterol synthesis, Pulmonary dysfunction, Relative organ weight",
author = "Mundy, {Dorothy I.} and Lopez, {Adam M.} and Posey, {Kenneth S.} and Chuang, {Jen Chieh} and Ramirez, {Charina M.} and Scherer, {Philipp E.} and Turley, {Stephen D.}",
year = "2014",
doi = "10.1016/j.bbalip.2014.04.002",
language = "English (US)",
volume = "1841",
pages = "995--1002",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
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number = "7",

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TY - JOUR

T1 - Impact of the loss of caveolin-1 on lung mass and cholesterol metabolism in mice with and without the lysosomal cholesterol transporter, Niemann-Pick type C1

AU - Mundy, Dorothy I.

AU - Lopez, Adam M.

AU - Posey, Kenneth S.

AU - Chuang, Jen Chieh

AU - Ramirez, Charina M.

AU - Scherer, Philipp E.

AU - Turley, Stephen D.

PY - 2014

Y1 - 2014

N2 - Caveolin-1 (Cav-1) is a major structural protein in caveolae in the plasma membranes of many cell types, particularly endothelial cells and adipocytes. Loss of Cav-1 function has been implicated in multiple diseases affecting the cardiopulmonary and central nervous systems, as well as in specific aspects of sterol and lipid metabolism in the liver and intestine. Lungs contain an exceptionally high level of Cav-1. Parameters of cholesterol metabolism in the lung were measured, initially in Cav-1-deficient mice (Cav-1-/-), and subsequently in Cav-1-/- mice that also lacked the lysosomal cholesterol transporter Niemann-Pick C1 (Npc1) (Cav-1-/-:Npc1 -/-). In 50-day-old Cav-1-/- mice fed a low- or high-cholesterol chow diet, the total cholesterol concentration (mg/g) in the lungs was marginally lower than in the Cav-1+/+ controls, but due to an expansion in their lung mass exceeding 30%, whole-lung cholesterol content (mg/organ) was moderately elevated. Lung mass (g) in the Cav-1 -/-:Npc1-/- mice (0.356 ± 0.022) markedly exceeded that in their Cav-1+/+:Npc1+/+ controls (0.137 ± 0.009), as well as in their Cav-1-/-:Npc1+/+ (0.191 ± 0.013) and Cav-1+/+:Npc1-/- (0.213 ± 0.022) littermates. The corresponding lung total cholesterol contents (mg/organ) in mice of these genotypes were 6.74 ± 0.17, 0.71 ± 0.05, 0.96 ± 0.05 and 3.12 ± 0.43, respectively, with the extra cholesterol in the Cav-1-/-:Npc1-/- and Cav-1+/+:Npc1 -/- mice being nearly all unesterified (UC). The exacerbation of the Npc1 lung phenotype and increase in the UC level in the Cav-1 -/-:Npc1-/- mice imply a regulatory role of Cav-1 in pulmonary cholesterol metabolism when lysosomal sterol transport is disrupted.

AB - Caveolin-1 (Cav-1) is a major structural protein in caveolae in the plasma membranes of many cell types, particularly endothelial cells and adipocytes. Loss of Cav-1 function has been implicated in multiple diseases affecting the cardiopulmonary and central nervous systems, as well as in specific aspects of sterol and lipid metabolism in the liver and intestine. Lungs contain an exceptionally high level of Cav-1. Parameters of cholesterol metabolism in the lung were measured, initially in Cav-1-deficient mice (Cav-1-/-), and subsequently in Cav-1-/- mice that also lacked the lysosomal cholesterol transporter Niemann-Pick C1 (Npc1) (Cav-1-/-:Npc1 -/-). In 50-day-old Cav-1-/- mice fed a low- or high-cholesterol chow diet, the total cholesterol concentration (mg/g) in the lungs was marginally lower than in the Cav-1+/+ controls, but due to an expansion in their lung mass exceeding 30%, whole-lung cholesterol content (mg/organ) was moderately elevated. Lung mass (g) in the Cav-1 -/-:Npc1-/- mice (0.356 ± 0.022) markedly exceeded that in their Cav-1+/+:Npc1+/+ controls (0.137 ± 0.009), as well as in their Cav-1-/-:Npc1+/+ (0.191 ± 0.013) and Cav-1+/+:Npc1-/- (0.213 ± 0.022) littermates. The corresponding lung total cholesterol contents (mg/organ) in mice of these genotypes were 6.74 ± 0.17, 0.71 ± 0.05, 0.96 ± 0.05 and 3.12 ± 0.43, respectively, with the extra cholesterol in the Cav-1-/-:Npc1-/- and Cav-1+/+:Npc1 -/- mice being nearly all unesterified (UC). The exacerbation of the Npc1 lung phenotype and increase in the UC level in the Cav-1 -/-:Npc1-/- mice imply a regulatory role of Cav-1 in pulmonary cholesterol metabolism when lysosomal sterol transport is disrupted.

KW - Cellular cholesterol trafficking

KW - Cholesterol feeding

KW - Cholesterol synthesis

KW - Pulmonary dysfunction

KW - Relative organ weight

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U2 - 10.1016/j.bbalip.2014.04.002

DO - 10.1016/j.bbalip.2014.04.002

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VL - 1841

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JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

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