Impact of transfusion of autologous 7-versus 42-day-old AS-3 red blood cells on tissue oxygenation and the microcirculation in healthy volunteers

Russell S. Roberson, Evelyn Lockhart, Nathan I. Shapiro, Nicholas Bandarenko, Timothy J. McMahon, Michael J. Massey, William D. White, Elliott Bennett-Guerrero

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Stored red blood cells (RBCs) accumulate biochemical and biophysical changes. Maximum storage duration is based on acceptable in vitro characteristics and 24-hour survival, but not RBC function. Relatively little is known about the impact of RBC storage duration on oxygenation and the microcirculation. Study design and methods: Eight healthy subjects donated a double RBC apheresis, which were prestorage leukoreduced and processed in AS-3. Subjects were transfused 1 unit of RBCs at 7 and 42 days after blood collection. Measurements of percentage of tissue oxygenation in the thenar eminence muscle (StO2) and brain (SctO2) were recorded with Food and Drug Administration-cleared noninvasive devices. Sublingual microvascular blood flow (microcirculatory flow index [MFI]) was quantified before and after RBC transfusion using a video microscope. Raw electronic data for all measurements were analyzed by a blinded observer at a core laboratory. Results: The only pre- versus posttransfusion change observed in measurements of SctO2, StO2, or MFI was a very small increase in SctO2, from 70.4 to 71.8 (means, p = 0.032) at 7 days. There was no significant difference in the amount of pre-post change at 7 days versus 42 days for any of the measures. Conclusion: Transfusion of 1 unit of 42-day-stored RBCs to healthy subjects has no overt detrimental effect on tissue oxygenation or the microcirculation assessed by clinically available monitors.

Original languageEnglish (US)
Pages (from-to)2459-2464
Number of pages6
JournalTransfusion
Volume52
Issue number11
DOIs
StatePublished - Nov 2012

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Microcirculation
Healthy Volunteers
Erythrocytes
Erythrocyte Transfusion
Blood Component Removal
United States Food and Drug Administration
Equipment and Supplies
Muscles
Brain

ASJC Scopus subject areas

  • Hematology
  • Immunology
  • Immunology and Allergy

Cite this

Roberson, R. S., Lockhart, E., Shapiro, N. I., Bandarenko, N., McMahon, T. J., Massey, M. J., ... Bennett-Guerrero, E. (2012). Impact of transfusion of autologous 7-versus 42-day-old AS-3 red blood cells on tissue oxygenation and the microcirculation in healthy volunteers. Transfusion, 52(11), 2459-2464. https://doi.org/10.1111/j.1537-2995.2012.03615.x

Impact of transfusion of autologous 7-versus 42-day-old AS-3 red blood cells on tissue oxygenation and the microcirculation in healthy volunteers. / Roberson, Russell S.; Lockhart, Evelyn; Shapiro, Nathan I.; Bandarenko, Nicholas; McMahon, Timothy J.; Massey, Michael J.; White, William D.; Bennett-Guerrero, Elliott.

In: Transfusion, Vol. 52, No. 11, 11.2012, p. 2459-2464.

Research output: Contribution to journalArticle

Roberson, RS, Lockhart, E, Shapiro, NI, Bandarenko, N, McMahon, TJ, Massey, MJ, White, WD & Bennett-Guerrero, E 2012, 'Impact of transfusion of autologous 7-versus 42-day-old AS-3 red blood cells on tissue oxygenation and the microcirculation in healthy volunteers', Transfusion, vol. 52, no. 11, pp. 2459-2464. https://doi.org/10.1111/j.1537-2995.2012.03615.x
Roberson, Russell S. ; Lockhart, Evelyn ; Shapiro, Nathan I. ; Bandarenko, Nicholas ; McMahon, Timothy J. ; Massey, Michael J. ; White, William D. ; Bennett-Guerrero, Elliott. / Impact of transfusion of autologous 7-versus 42-day-old AS-3 red blood cells on tissue oxygenation and the microcirculation in healthy volunteers. In: Transfusion. 2012 ; Vol. 52, No. 11. pp. 2459-2464.
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AU - Bandarenko, Nicholas

AU - McMahon, Timothy J.

AU - Massey, Michael J.

AU - White, William D.

AU - Bennett-Guerrero, Elliott

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