Impaired activation of tumoricidal function in macrophages from mammary tumor bearers: The role of IFN-γ

E. M. Sotomayor, X. F. Cheng, Y. X. Fu, A. Hawkins, C. Calderon-Higginson, D. M. Lopez

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The growth of the D1-DMBA-3 mammary adenocarcinoma in BALB/c mice results in a profound deficit in the tumoricidal function of their peritoneal elicited macrophages (PEM) after stimulation with lipopolysaccharide (LPS). The capacity of these macrophages to respond to stronger signals, such as a combination of LPS and low levels (5 U/ml) of interferon-gamma (IFN-γ) is also impaired. Importantly, a combination of high levels (50 U/ml) of IFN-γ with 10 μg/ml of LPS, is able to trigger a cytolytic response in macrophages from tumor bearing mice against mammary tumor target cells, indicating that their lytic machinery is intact. However, IFN-γ production is severely diminished in T lymphocytes from tumor bearing mice as detected by ELISA, moreover, Northern blots revealed that the levels of IFN-γ RNA are also decreased in T cells from tumor bearers. The cooperation between T cells and macrophages is mediated, at least in part, by IFN-γ. Thus, mammary tumors have the potential to overcome host defenses, either by affecting the capacity of macrophages to respond adequately to activating agents, and/or by impairing the production of T-cell derived lymphokines important in macrophage activation for tumor killing.

Original languageEnglish (US)
Pages (from-to)719-727
Number of pages9
JournalInternational journal of oncology
Volume3
Issue number4
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Sotomayor, E. M., Cheng, X. F., Fu, Y. X., Hawkins, A., Calderon-Higginson, C., & Lopez, D. M. (1993). Impaired activation of tumoricidal function in macrophages from mammary tumor bearers: The role of IFN-γ. International journal of oncology, 3(4), 719-727.