Impaired endothelial function and microvascular asymmetrical dimethylarginine in angiotensin II-infused rats: Effects of tempol

Dan Wang, Zaiming Luo, Xiaoyan Wang, Pedro A. Jose, J R Falck, William J. Welch, Shakil Aslam, Tom Teerlink, Christopher S. Wilcox

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Angiotensin (Ang) II causes endothelial dysfunction, which is associated with cardiovascular risk. We investigated the hypothesis that Ang II increases microvascular reactive oxygen species and asymmetrical dimethylarginine and switches endothelial function from vasodilator to vasoconstrictor pathways. Acetylcholine-induced endothelium-dependent responses of mesenteric resistance arterioles were assessed in a myograph and vascular NO and reactive oxygen species by fluorescent probes in groups (n=6) of male rats infused for 14 days with Ang II (200 ng/kg per minute) or given a sham infusion. Additional groups of Ang or sham-infused rats were given oral Tempol (2 mmol • L). Ang II infusion increased mean blood pressure (119±5 versus 89±7 mm Hg; P<0.005) and plasma malondialdehyde (0.57±0.02 versus 0.37±0.05 μmol • L; P<0.035) and decreased maximal endothelium-dependent relaxation (18±5% versus 54±6%; P<0.005) and hyperpolarizing (19±3% versus 29±3%; P<0.05) responses and NO activity (0.9±0.1 versus 1.6±0.2 U; P<0.01) yet enhanced endothelium-dependent contraction responses (23±5% versus 5±5%; P<0.05) and reactive oxygen species production (0.82±0.05 versus 0.15±0.03 U; P<0.01). Ang II decreased the expression of dimethylarginine dimethylaminohydrolase 2 and increased asymmetrical dimethylarginine in vessels (450±50 versus 260±35 pmol/mg of protein; P<0.01) but not plasma. Tempol prevented any significant changes with Ang II. In conclusion, Ang redirected endothelial responses from relaxation to contraction, reduced vascular NO, and increased asymmetrical dimethylarginine. These effects were dependent on reactive oxygen species and could, therefore, be targeted with effective antioxidant therapy.

Original languageEnglish (US)
Pages (from-to)950-955
Number of pages6
JournalHypertension
Volume56
Issue number5
DOIs
StatePublished - Nov 1 2010

Keywords

  • NO
  • dimethylarginine dimethylaminohydrolase
  • endothelial dysfunction
  • hypertension
  • reactive oxygen species

ASJC Scopus subject areas

  • Internal Medicine

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