Impairment of flow-induced dilation of skeletal muscle arterioles with elevated oxygen in normotensive and hypertensive rats

Jefferson C. Frisbee, Richard J. Roman, J R Falck, J. Russell Linderman, Julian H. Lombard

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The effects of elevated PO2 on flow-induced dilation of in situ skeletal muscle arterioles was assessed in cremaster muscle preparations from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Blood flow increases in selected arterioles were initiated by occlusion of a parallel daughter branch from a parent arteriole. Changes in the diameter of the perfused arteriole were measured with a video micrometer and erythrocyte velocity was measured using optical Doppler velocimetry. Superfusate PO2 was controlled by changing the O2 concentration (0% O2 or 21% O2) of the equilibration gas mixture. The increase in arteriolar diameter during occlusion was reduced in SHR compared to WKY rats, resulting in an elevated wall shear rate in SHR. Elevated PO2 decreased flow-induced dilation in both groups and increased wall shear rate during parallel occlusion. An inhibitor of the formation of 20-HETE via cytochrome P450-4A enzymes (P450), dibromododecenyl methylsulfimide, minimized O2-induced constriction of arterioles and prevented the O2-induced decrease in flow-induced dilation and the increase in wall shear rate in both SHR and WKY rats. These results suggest that: (1) flow-induced dilation of in situ skeletal muscle arterioles is impaired in SHR compared to WKY, (2) elevated O2 compromises flow-induced dilation in both groups, (3) 20-HETE contributes to both the O2-induced increases in resting tone and the reduced flow-induced dilation of cremasteric arterioles with elevated PO2. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)37-48
Number of pages12
JournalMicrovascular Research
Volume60
Issue number1
DOIs
StatePublished - Jul 2000

Fingerprint

Arterioles
Muscle
Rats
Dilatation
Skeletal Muscle
Inbred SHR Rats
Oxygen
Inbred WKY Rats
Shear deformation
Cytochrome P-450 Enzyme System
Abdominal Muscles
Rheology
Constriction
Gas mixtures
Velocity measurement
Erythrocytes
Gases
Blood

Keywords

  • 20- HETE
  • Cremaster muscle
  • Cytochrome P450
  • DDMS
  • Dibromododecenyl methylsulfimide
  • Microcirculation
  • Shear stress
  • Spontaneous hypertension
  • Wall shear rate

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Impairment of flow-induced dilation of skeletal muscle arterioles with elevated oxygen in normotensive and hypertensive rats. / Frisbee, Jefferson C.; Roman, Richard J.; Falck, J R; Linderman, J. Russell; Lombard, Julian H.

In: Microvascular Research, Vol. 60, No. 1, 07.2000, p. 37-48.

Research output: Contribution to journalArticle

Frisbee, Jefferson C. ; Roman, Richard J. ; Falck, J R ; Linderman, J. Russell ; Lombard, Julian H. / Impairment of flow-induced dilation of skeletal muscle arterioles with elevated oxygen in normotensive and hypertensive rats. In: Microvascular Research. 2000 ; Vol. 60, No. 1. pp. 37-48.
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