Abstract
A new amplification technique is described for the detection of small amounts of antigen in ethanol‐fixed cytologic specimens and formalin‐fixed tissues. By adding a third antibody layer to the already sensitive avidin‐biotin immunoperoxidase system. we have demonstrated a considerable increase in both the quantitative and qualitative aspects of this immunostaining method. In evaluating this technique, we employed a previously defined small cell lung carcinoma cell surface antigen system and monoclonal antibodies.
Original language | English (US) |
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Pages (from-to) | 133-136 |
Number of pages | 4 |
Journal | Diagnostic cytopathology |
Volume | 1 |
Issue number | 2 |
DOIs | |
State | Published - 1985 |
Keywords
- Avidin‐biotin complex method
- Immunocytochemistry
- Immunoperoxidase
- Monoclonal antibodies
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology