Improved immunodetection of human papillomavirus E7

Ju Hong Jeon, Sung Yup Cho, Chai Wan Kim, Dong Myung Shin, Joon Cheol Kwon, Kyung Ho Choi, In Gyu Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human papillomavirus E7 (HPV E7) is a viral oncoprotein that plays an important role in cervical carcinogenesis through binding with retinoblastoma protein (Rb). Inactivation of Rb by E7 is necessary but not sufficient for cellular transformation, suggesting other protein-protein interactions are required for E7-mediated cellular transformation aside from the interaction with Rb. However, studies on the oncogenic function of HPV E7 have been limited by its poor immunoreactivity. In this report, we show that the fixation of purified recombinant HPV E7 on blotted nitrocellulose membrane with glutaldehyde markedly enhanced the immunoreactivity of HPV E7 protein. Using HeLa and Caski cell line which are infected with HPV 18 and HPV 16, respectively, we demonstrated that native HPV E7 proteins also could be detected by this method. These results therefore can provide the experimental conditions for detection of HPV E7 proteins with greater sensitivity and may help to analyze E7 functions.

Original languageEnglish (US)
Pages (from-to)496-499
Number of pages4
JournalExperimental and Molecular Medicine
Volume34
Issue number6
StatePublished - Dec 31 2002

Fingerprint

Papillomavirus E7 Proteins
Retinoblastoma Protein
Collodion
Oncogene Proteins
Retinoblastoma Binding Proteins
Proteins
Cells
Membranes
Human papillomavirus 18
Human papillomavirus 16
HeLa Cells
Carcinogenesis
Cell Line

Keywords

  • Glutaral
  • Immunoblotting
  • Immunologic techniques
  • Oncogene proteins, viral, papillomavirus, human

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Jeon, J. H., Cho, S. Y., Kim, C. W., Shin, D. M., Kwon, J. C., Choi, K. H., & Kim, I. G. (2002). Improved immunodetection of human papillomavirus E7. Experimental and Molecular Medicine, 34(6), 496-499.

Improved immunodetection of human papillomavirus E7. / Jeon, Ju Hong; Cho, Sung Yup; Kim, Chai Wan; Shin, Dong Myung; Kwon, Joon Cheol; Choi, Kyung Ho; Kim, In Gyu.

In: Experimental and Molecular Medicine, Vol. 34, No. 6, 31.12.2002, p. 496-499.

Research output: Contribution to journalArticle

Jeon, JH, Cho, SY, Kim, CW, Shin, DM, Kwon, JC, Choi, KH & Kim, IG 2002, 'Improved immunodetection of human papillomavirus E7', Experimental and Molecular Medicine, vol. 34, no. 6, pp. 496-499.
Jeon JH, Cho SY, Kim CW, Shin DM, Kwon JC, Choi KH et al. Improved immunodetection of human papillomavirus E7. Experimental and Molecular Medicine. 2002 Dec 31;34(6):496-499.
Jeon, Ju Hong ; Cho, Sung Yup ; Kim, Chai Wan ; Shin, Dong Myung ; Kwon, Joon Cheol ; Choi, Kyung Ho ; Kim, In Gyu. / Improved immunodetection of human papillomavirus E7. In: Experimental and Molecular Medicine. 2002 ; Vol. 34, No. 6. pp. 496-499.
@article{a92f956d542f4f818bd646e43c8e76c3,
title = "Improved immunodetection of human papillomavirus E7",
abstract = "Human papillomavirus E7 (HPV E7) is a viral oncoprotein that plays an important role in cervical carcinogenesis through binding with retinoblastoma protein (Rb). Inactivation of Rb by E7 is necessary but not sufficient for cellular transformation, suggesting other protein-protein interactions are required for E7-mediated cellular transformation aside from the interaction with Rb. However, studies on the oncogenic function of HPV E7 have been limited by its poor immunoreactivity. In this report, we show that the fixation of purified recombinant HPV E7 on blotted nitrocellulose membrane with glutaldehyde markedly enhanced the immunoreactivity of HPV E7 protein. Using HeLa and Caski cell line which are infected with HPV 18 and HPV 16, respectively, we demonstrated that native HPV E7 proteins also could be detected by this method. These results therefore can provide the experimental conditions for detection of HPV E7 proteins with greater sensitivity and may help to analyze E7 functions.",
keywords = "Glutaral, Immunoblotting, Immunologic techniques, Oncogene proteins, viral, papillomavirus, human",
author = "Jeon, {Ju Hong} and Cho, {Sung Yup} and Kim, {Chai Wan} and Shin, {Dong Myung} and Kwon, {Joon Cheol} and Choi, {Kyung Ho} and Kim, {In Gyu}",
year = "2002",
month = "12",
day = "31",
language = "English (US)",
volume = "34",
pages = "496--499",
journal = "Experimental and Molecular Medicine",
issn = "1226-3613",
publisher = "Korean Society of Med. Biochemistry and Mol. Biology",
number = "6",

}

TY - JOUR

T1 - Improved immunodetection of human papillomavirus E7

AU - Jeon, Ju Hong

AU - Cho, Sung Yup

AU - Kim, Chai Wan

AU - Shin, Dong Myung

AU - Kwon, Joon Cheol

AU - Choi, Kyung Ho

AU - Kim, In Gyu

PY - 2002/12/31

Y1 - 2002/12/31

N2 - Human papillomavirus E7 (HPV E7) is a viral oncoprotein that plays an important role in cervical carcinogenesis through binding with retinoblastoma protein (Rb). Inactivation of Rb by E7 is necessary but not sufficient for cellular transformation, suggesting other protein-protein interactions are required for E7-mediated cellular transformation aside from the interaction with Rb. However, studies on the oncogenic function of HPV E7 have been limited by its poor immunoreactivity. In this report, we show that the fixation of purified recombinant HPV E7 on blotted nitrocellulose membrane with glutaldehyde markedly enhanced the immunoreactivity of HPV E7 protein. Using HeLa and Caski cell line which are infected with HPV 18 and HPV 16, respectively, we demonstrated that native HPV E7 proteins also could be detected by this method. These results therefore can provide the experimental conditions for detection of HPV E7 proteins with greater sensitivity and may help to analyze E7 functions.

AB - Human papillomavirus E7 (HPV E7) is a viral oncoprotein that plays an important role in cervical carcinogenesis through binding with retinoblastoma protein (Rb). Inactivation of Rb by E7 is necessary but not sufficient for cellular transformation, suggesting other protein-protein interactions are required for E7-mediated cellular transformation aside from the interaction with Rb. However, studies on the oncogenic function of HPV E7 have been limited by its poor immunoreactivity. In this report, we show that the fixation of purified recombinant HPV E7 on blotted nitrocellulose membrane with glutaldehyde markedly enhanced the immunoreactivity of HPV E7 protein. Using HeLa and Caski cell line which are infected with HPV 18 and HPV 16, respectively, we demonstrated that native HPV E7 proteins also could be detected by this method. These results therefore can provide the experimental conditions for detection of HPV E7 proteins with greater sensitivity and may help to analyze E7 functions.

KW - Glutaral

KW - Immunoblotting

KW - Immunologic techniques

KW - Oncogene proteins, viral, papillomavirus, human

UR - http://www.scopus.com/inward/record.url?scp=0037207238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037207238&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 496

EP - 499

JO - Experimental and Molecular Medicine

JF - Experimental and Molecular Medicine

SN - 1226-3613

IS - 6

ER -