Improved immunodetection of human papillomavirus E7

Ju Hong Jeon, Sung Yup Cho, Chai Wan Kim, Dong Myung Shin, Joon Cheol Kwon, Kyung Ho Choi, In Gyu Kim

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Human papillomavirus E7 (HPV E7) is a viral oncoprotein that plays an important role in cervical carcinogenesis through binding with retinoblastoma protein (Rb). Inactivation of Rb by E7 is necessary but not sufficient for cellular transformation, suggesting other protein-protein interactions are required for E7-mediated cellular transformation aside from the interaction with Rb. However, studies on the oncogenic function of HPV E7 have been limited by its poor immunoreactivity. In this report, we show that the fixation of purified recombinant HPV E7 on blotted nitrocellulose membrane with glutaldehyde markedly enhanced the immunoreactivity of HPV E7 protein. Using HeLa and Caski cell line which are infected with HPV 18 and HPV 16, respectively, we demonstrated that native HPV E7 proteins also could be detected by this method. These results therefore can provide the experimental conditions for detection of HPV E7 proteins with greater sensitivity and may help to analyze E7 functions.

Original languageEnglish (US)
Pages (from-to)496-499
Number of pages4
JournalExperimental and Molecular Medicine
Volume34
Issue number6
DOIs
StatePublished - Dec 31 2002

Keywords

  • Glutaral
  • Immunoblotting
  • Immunologic techniques
  • Oncogene proteins, viral, papillomavirus, human

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Improved immunodetection of human papillomavirus E7'. Together they form a unique fingerprint.

Cite this