Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions

Xiaoyu Wang, Ying Dong, Ameena J. Jiwani, Yonglong Zou, Johanne Pastor, Makoto Kuro-O, Amyn A. Habib, Minzi Ruan, David A. Boothman, Chin Rang Yang

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.

Original languageEnglish (US)
Article number53
JournalProteome Science
Volume9
DOIs
StatePublished - Sep 15 2011

Keywords

  • Analysis
  • Cross-talkimage
  • Glioma
  • Protein array
  • Quantitative analysis
  • Signaling pathway
  • Systems biology
  • quantum dot

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Wang, X., Dong, Y., Jiwani, A. J., Zou, Y., Pastor, J., Kuro-O, M., Habib, A. A., Ruan, M., Boothman, D. A., & Yang, C. R. (2011). Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions. Proteome Science, 9, [53]. https://doi.org/10.1186/1477-5956-9-53