Improving sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

H. M. Santos, R. Rial-Otero, L. Fernandes, G. Vale, M. G. Rivas, I. Moura, J. L. Capelo

Research output: Contribution to journalReview article

23 Scopus citations

Abstract

Three ultrasonic energy sources were studied to speed up the sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein reduction, alkylation, and enzymatic digestion steps were done in 15 min. Nine proteins, including zinc resistance-associated protein precursor from Desulfovibrio desulfuricans strain G20 and split-soret cytochrome c from D. desulfuricans ATCC27774 were successfully identified with the new protocol.

Original languageEnglish (US)
Pages (from-to)3393-3399
Number of pages7
JournalJournal of Proteome Research
Volume6
Issue number9
DOIs
StatePublished - Sep 1 2007

Keywords

  • In-solution protein digestion
  • MALDI-TOF-MS
  • Protein identification
  • Sonoreactor
  • Ultrasonic bath
  • Ultrasonic probe

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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