In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells

Shuo Gao, Lei Zhao, Zhiqiang Fan, Vikram D. Kodibagkar, Li Liu, Hanqin Wang, Hong Xu, Mingli Tu, Bifu Hu, Chuanbin Cao, Zhenjian Zhang, Jian Xin Yu

Research output: Contribution to journalArticlepeer-review

Abstract

For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T1 and T2 in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T1 and T2 relaxation mapping.

Original languageEnglish (US)
Article number709581
JournalFrontiers in Chemistry
Volume9
DOIs
StatePublished - Jul 15 2021

Keywords

  • in vitro H-MRI imaging
  • lacZ gene reporter
  • responsive Fe-based H-MRI agent
  • synthesis
  • T and T relaxation mapping
  • β-galactosidase detection

ASJC Scopus subject areas

  • Chemistry(all)

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